Electropolymerized bioresistant coatings of OEGylated dendron carbazoles: Design parameters and protein resistance SPR studies

Mary Jane Felipe, Pampa Dutta, Roderick Pernites, Ramakrishna Ponnapati, Rigoberto C. Advincula

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The electrografting of oligoethylene glycol or (OEG)ylated carbazole linear dendrons and their protein adsorption resistance properties have been investigated by surface plasmon resonance (SPR) spectroscopy. A series of the carbazole dendron generations, G 0, G 1, G 2 were synthesized and electrodeposited by cyclic voltammetry (CV) on Au-glass substrate which also served as a surface for evanescent waveguide excitation in SPR. In addition, the films were characterized by in-situ electrochemical SPR (EC-SPR), static water contact angle, and X-ray photoelectron spectroscopy (XPS) measurements. It was observed that electropolymerized films prepared from the higher generation linear-dendron G 2, is most effective in preventing non-specific protein adsorption as observed by SPR kinetic measurements using fibrinogen as model protein. Film thickness also played a critical role in protein adsorption resistance - electrodeposition approaching monolayer thickness gave the highest protein resistance. In addition, the films were evaluated for non-specific protein binding against the smaller proteins, lysozyme and bovine serum albumin (BSA). The study provides insight to manipulating the architecture and composition of protein resistant materials deposited on metals and semi-conducting substrates and their possible use in biomedical applications.

Original languageEnglish
Pages (from-to)427-437
Number of pages11
JournalPolymer
Volume53
Issue number2
DOIs
Publication statusPublished - 2012 Jan 24
Externally publishedYes

Fingerprint

Carbazoles
Surface plasmon resonance
Proteins
Coatings
Adsorption
Dendrimers
Glycols
Substrates
Muramidase
Bovine Serum Albumin
dendron
Electrodeposition
Fibrinogen
Cyclic voltammetry
Contact angle
Film thickness
Monolayers
Waveguides
X ray photoelectron spectroscopy
Enzymes

Keywords

  • Dendrons
  • Protein-resistance
  • SPR

ASJC Scopus subject areas

  • Polymers and Plastics
  • Organic Chemistry

Cite this

Electropolymerized bioresistant coatings of OEGylated dendron carbazoles : Design parameters and protein resistance SPR studies. / Felipe, Mary Jane; Dutta, Pampa; Pernites, Roderick; Ponnapati, Ramakrishna; Advincula, Rigoberto C.

In: Polymer, Vol. 53, No. 2, 24.01.2012, p. 427-437.

Research output: Contribution to journalArticle

Felipe, Mary Jane ; Dutta, Pampa ; Pernites, Roderick ; Ponnapati, Ramakrishna ; Advincula, Rigoberto C. / Electropolymerized bioresistant coatings of OEGylated dendron carbazoles : Design parameters and protein resistance SPR studies. In: Polymer. 2012 ; Vol. 53, No. 2. pp. 427-437.
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