Electrostatic layer-by-layer construction of fibrous TMV biofilms

Brylee David B. Tiu, Daniel L. Kernan, Sicily B. Tiu, Amy M. Wen, Yi Zheng, Jonathan K. Pokorski, Rigoberto C. Advincula*, Nicole F. Steinmetz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

As nature's choice in designing complex architectures, the bottom-up assembly of nanoscale building blocks offers unique solutions in achieving more complex and smaller morphologies with wide-ranging applications in medicine, energy, and materials science as compared to top-down manufacturing. In this work, we employ charged tobacco mosaic virus (TMV-wt and TMV-lys) nanoparticles in constructing multilayered fibrous networks via electrostatic layer-by-layer (LbL) deposition. In neutral aqueous media, TMV-wt assumes an anionic surface charge. TMV-wt was paired with a genetically engineered TMV-lys variant that displays a corona of lysine side chains on its solvent-exposed surface. The electrostatic interaction between TMV-wt and TMV-lys nanoparticles became the driving force in the highly controlled buildup of the multilayer TMV constructs. Since the resulting morphology closely resembles the 3-dimensional fibrous network of an extracellular matrix (ECM), the capability of the TMV assemblies to support the adhesion of NIH-3T3 fibroblast cells was investigated, demonstrating potential utility in regenerative medicine. Lastly, the layer-by-layer deposition was extended to release the TMV scaffolds as free-standing biomembranes. To demonstrate potential application in drug delivery or vaccine technology, cargo-functionalized TMV biofilms were programmed.

Original languageEnglish
Pages (from-to)1580-1590
Number of pages11
JournalNanoscale
Volume9
Issue number4
DOIs
Publication statusPublished - 2017 Jan 28
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)

Fingerprint

Dive into the research topics of 'Electrostatic layer-by-layer construction of fibrous TMV biofilms'. Together they form a unique fingerprint.

Cite this