Electrostatic layer-by-layer construction of fibrous TMV biofilms

Brylee David B. Tiu, Daniel L. Kernan, Sicily B. Tiu, Amy M. Wen, Yi Zheng, Jonathan K. Pokorski, Rigoberto C. Advincula, Nicole F. Steinmetz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

As nature's choice in designing complex architectures, the bottom-up assembly of nanoscale building blocks offers unique solutions in achieving more complex and smaller morphologies with wide-ranging applications in medicine, energy, and materials science as compared to top-down manufacturing. In this work, we employ charged tobacco mosaic virus (TMV-wt and TMV-lys) nanoparticles in constructing multilayered fibrous networks via electrostatic layer-by-layer (LbL) deposition. In neutral aqueous media, TMV-wt assumes an anionic surface charge. TMV-wt was paired with a genetically engineered TMV-lys variant that displays a corona of lysine side chains on its solvent-exposed surface. The electrostatic interaction between TMV-wt and TMV-lys nanoparticles became the driving force in the highly controlled buildup of the multilayer TMV constructs. Since the resulting morphology closely resembles the 3-dimensional fibrous network of an extracellular matrix (ECM), the capability of the TMV assemblies to support the adhesion of NIH-3T3 fibroblast cells was investigated, demonstrating potential utility in regenerative medicine. Lastly, the layer-by-layer deposition was extended to release the TMV scaffolds as free-standing biomembranes. To demonstrate potential application in drug delivery or vaccine technology, cargo-functionalized TMV biofilms were programmed.

Original languageEnglish
Pages (from-to)1580-1590
Number of pages11
JournalNanoscale
Volume9
Issue number4
DOIs
Publication statusPublished - 2017 Jan 28
Externally publishedYes

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Biofilms
Electrostatics
Nanoparticles
Vaccines
Tobacco
Materials science
Fibroblasts
Surface charge
Coulomb interactions
Drug delivery
Viruses
Scaffolds
Lysine
Medicine
Multilayers
Adhesion
Cells
Regenerative Medicine

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Tiu, B. D. B., Kernan, D. L., Tiu, S. B., Wen, A. M., Zheng, Y., Pokorski, J. K., ... Steinmetz, N. F. (2017). Electrostatic layer-by-layer construction of fibrous TMV biofilms. Nanoscale, 9(4), 1580-1590. https://doi.org/10.1039/c6nr06266k

Electrostatic layer-by-layer construction of fibrous TMV biofilms. / Tiu, Brylee David B.; Kernan, Daniel L.; Tiu, Sicily B.; Wen, Amy M.; Zheng, Yi; Pokorski, Jonathan K.; Advincula, Rigoberto C.; Steinmetz, Nicole F.

In: Nanoscale, Vol. 9, No. 4, 28.01.2017, p. 1580-1590.

Research output: Contribution to journalArticle

Tiu, BDB, Kernan, DL, Tiu, SB, Wen, AM, Zheng, Y, Pokorski, JK, Advincula, RC & Steinmetz, NF 2017, 'Electrostatic layer-by-layer construction of fibrous TMV biofilms', Nanoscale, vol. 9, no. 4, pp. 1580-1590. https://doi.org/10.1039/c6nr06266k
Tiu BDB, Kernan DL, Tiu SB, Wen AM, Zheng Y, Pokorski JK et al. Electrostatic layer-by-layer construction of fibrous TMV biofilms. Nanoscale. 2017 Jan 28;9(4):1580-1590. https://doi.org/10.1039/c6nr06266k
Tiu, Brylee David B. ; Kernan, Daniel L. ; Tiu, Sicily B. ; Wen, Amy M. ; Zheng, Yi ; Pokorski, Jonathan K. ; Advincula, Rigoberto C. ; Steinmetz, Nicole F. / Electrostatic layer-by-layer construction of fibrous TMV biofilms. In: Nanoscale. 2017 ; Vol. 9, No. 4. pp. 1580-1590.
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