Elevation of plasma prolactin concentrations by low temperature is the cause of spermatogonial cell death in the newt, Cynops pyrrhogaster

Takashi Yazawa, Kazutoshi Yamamoto, Sakae Kikuyama, Shin Ichi Abé

    Research output: Contribution to journalArticle

    34 Citations (Scopus)

    Abstract

    Temperature plays an important role in reproduction of urodeles. Spermatogenesis in newts is arrested when the environmental temperature lowers. We found that transfer of newts, Cynops pyrrhogaster, to low temperature (8 and 12°C) caused cell death of spermatogonia just before meiosis and elevation of prolaetin concentration in the newt plasma. Injection of a dopamine antagonist (pimozide), which is known to increase the plasma prolactin concentration, to the newt caused significant increase of spermatogonial degeneration, whereas treatment with an agonist (bromocryptin), which is known to decrease the prolactin concentration, suppressed the cell death. Finally, injection of anti-prolactin serum into the newts which had been transferred to low temperature almost completely inhibited the spermatogonial degeneration for as long as 3 days. These results demonstrate that low temperature caused elevation of prolactin concentration in the newt blood, which induced cell death of spermatogonia just before meiosis.

    Original languageEnglish
    Pages (from-to)302-311
    Number of pages10
    JournalGeneral and Comparative Endocrinology
    Volume113
    Issue number2
    DOIs
    Publication statusPublished - 1999 Feb

    Fingerprint

    Salamandridae
    prolactin
    salamanders and newts
    Prolactin
    cell death
    Cell Death
    Temperature
    Spermatogonia
    temperature
    spermatogonia
    Meiosis
    meiosis
    pimozide
    Pimozide
    injection
    Injections
    Bromocriptine
    Dopamine Antagonists
    Spermatogenesis
    spermatogenesis

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Elevation of plasma prolactin concentrations by low temperature is the cause of spermatogonial cell death in the newt, Cynops pyrrhogaster. / Yazawa, Takashi; Yamamoto, Kazutoshi; Kikuyama, Sakae; Abé, Shin Ichi.

    In: General and Comparative Endocrinology, Vol. 113, No. 2, 02.1999, p. 302-311.

    Research output: Contribution to journalArticle

    Yazawa, Takashi ; Yamamoto, Kazutoshi ; Kikuyama, Sakae ; Abé, Shin Ichi. / Elevation of plasma prolactin concentrations by low temperature is the cause of spermatogonial cell death in the newt, Cynops pyrrhogaster. In: General and Comparative Endocrinology. 1999 ; Vol. 113, No. 2. pp. 302-311.
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