Elongated nanoparticle aggregates in cancer cells for mechanical destruction with low frequency rotating magnetic field

Yajing Shen, Congyu Wu, Taro Q.P. Uyeda, Gustavo R. Plaza, Bin Liu, Yu Han, Maciej S. Lesniak, Yu Cheng

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Magnetic nanoparticles (MNPs) functionalized with targeting moieties can recognize specific cell components and induce mechanical actuation under magnetic field. Their size is adequate for reaching tumors and targeting cancer cells. However, due to the nanometric size, the force generated by MNPs is smaller than the force required for largely disrupting key components of cells. Here, we show the magnetic assembly process of the nanoparticles inside the cells, to form elongated aggregates with the size required to produce elevated mechanical forces. We synthesized iron oxide nanoparticles doped with zinc, to obtain high magnetization, and functionalized with the epidermal growth factor (EGF) peptide for targeting cancer cells. Under a low frequency rotating magnetic field at 15 Hz and 40 mT, the internalized EGF-MNPs formed elongated aggregates and generated hundreds of pN to dramatically damage the plasma and lysosomal membranes. The physical disruption, including leakage of lysosomal hydrolases into the cytosol, led to programmed cell death and necrosis. Our work provides a novel strategy of designing magnetic nanomedicines for mechanical destruction of cancer cells.

Original languageEnglish
Pages (from-to)1735-1748
Number of pages14
JournalTheranostics
Volume7
Issue number6
DOIs
Publication statusPublished - 2017

Keywords

  • Brain cancer cells
  • Functionalized magnetic nanoparticles
  • Lysosome damage
  • Magneto-mechanical actuation
  • Plasma membrane damage

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

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