Elongation factor G is a critical target during oxidative damage to the translation system of Escherichia coli

Takanori Nagano, Kouji Kojima, Toru Hisabori, Hidenori Hayashi, Eugene Hayato Morita, Takashi Kanamori, Tomoko Miyagi, Takuya Ueda, Yoshitaka Nishiyama

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Elongation factor G (EF-G), a key protein in translational elongation, is known to be particularly susceptible to oxidation in Escherichia coli. However, neither the mechanism of the oxidation of EF-G nor the influence of its oxidation on translation is fully understood. In the present study, we investigated the effects of oxidants on the chemical properties and function of EF-G using a translation system in vitro derived from E. coli. Treatment of EF-G with 0.5 mM H 2O 2 resulted in the complete loss of translational activity. The inactivation of EF-G by H 2O 2 was attributable to the oxidation of two specific cysteine residues, namely, Cys 114 and Cys 266, and subsequent formation of an intramolecular disulfide bond. Replacement of Cys 114 by serine rendered EF-G insensitive to oxidation and inactivation by H 2O 2. Furthermore, generation of the translation system in vitro with the mutated EF-G protected the entire translation system from oxidation, suggesting that EF-G might be a primary target of oxidation within the translation system. Oxidized EF-G was reactivated via reduction of the disulfide bond by thioredoxin, a ubiquitous protein that mediates dithiol-disulfide exchange. Our observations indicate that the translational machinery in E. coli is regulated, in part, by the redox state of EF-G, which might depend on the balance between the supply of reducing power and the degree of oxidative stress.

Original languageEnglish
Pages (from-to)28697-28704
Number of pages8
JournalJournal of Biological Chemistry
Volume287
Issue number34
DOIs
Publication statusPublished - 2012 Aug 17
Externally publishedYes

Fingerprint

Peptide Elongation Factor G
Escherichia coli
Oxidation
Disulfides
Translational Peptide Chain Elongation
Electric Power Supplies
Thioredoxins
Oxidative stress
Oxidants
Serine
Chemical properties
Oxidation-Reduction
Machinery
Cysteine
Elongation
Proteins
Oxidative Stress

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Nagano, T., Kojima, K., Hisabori, T., Hayashi, H., Morita, E. H., Kanamori, T., ... Nishiyama, Y. (2012). Elongation factor G is a critical target during oxidative damage to the translation system of Escherichia coli. Journal of Biological Chemistry, 287(34), 28697-28704. https://doi.org/10.1074/jbc.M112.378067

Elongation factor G is a critical target during oxidative damage to the translation system of Escherichia coli. / Nagano, Takanori; Kojima, Kouji; Hisabori, Toru; Hayashi, Hidenori; Morita, Eugene Hayato; Kanamori, Takashi; Miyagi, Tomoko; Ueda, Takuya; Nishiyama, Yoshitaka.

In: Journal of Biological Chemistry, Vol. 287, No. 34, 17.08.2012, p. 28697-28704.

Research output: Contribution to journalArticle

Nagano, T, Kojima, K, Hisabori, T, Hayashi, H, Morita, EH, Kanamori, T, Miyagi, T, Ueda, T & Nishiyama, Y 2012, 'Elongation factor G is a critical target during oxidative damage to the translation system of Escherichia coli', Journal of Biological Chemistry, vol. 287, no. 34, pp. 28697-28704. https://doi.org/10.1074/jbc.M112.378067
Nagano, Takanori ; Kojima, Kouji ; Hisabori, Toru ; Hayashi, Hidenori ; Morita, Eugene Hayato ; Kanamori, Takashi ; Miyagi, Tomoko ; Ueda, Takuya ; Nishiyama, Yoshitaka. / Elongation factor G is a critical target during oxidative damage to the translation system of Escherichia coli. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 34. pp. 28697-28704.
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