Empirical study on grouping behavior of individual endothelial cells under shear stress

Kazuo Tanishita, Kazuhiro Nagayama, Madoka Fujii, Susumu Kudou

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

It has been recognized that the structure and function of the endothelial monolayer is affected by the applied mechanical stress. Microscopic observation of changes in cell morphology during the imposition of shear stress is necessary to identify the specific mechanism which mediates the cellular response to shear stress. In this study we observed the morphology and migration of individual cultured cells with the wall shear stress of 3 and 5 Pa under an optical microscope. We traced the particular group of cells under view, and examined the behavior of these particular cells during the imposition of shear stress. It was concluded that the initial orientation and shape of cells affect the process of receiving the external shear stress stimulus. The behavior of the individual cell depends on the geometry of surrounding cells. Thus, the external shear stress stimulus results in the "small grouping behavior" , primarily due to the biological and mechanical interaction among cells.

Original languageEnglish
Pages (from-to)715-720
Number of pages6
JournalJSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing
Volume42
Issue number3
Publication statusPublished - 1999 Sep
Externally publishedYes

Fingerprint

Endothelial cells
Shear stress
Cells
Monolayers
Microscopes
Geometry

Keywords

  • Bio-Fluid Mechanics
  • Biological Engineering
  • Biomechanics
  • Cell Morphology
  • Endothelial Cell

ASJC Scopus subject areas

  • Industrial and Manufacturing Engineering
  • Mechanical Engineering
  • Engineering(all)

Cite this

Empirical study on grouping behavior of individual endothelial cells under shear stress. / Tanishita, Kazuo; Nagayama, Kazuhiro; Fujii, Madoka; Kudou, Susumu.

In: JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing, Vol. 42, No. 3, 09.1999, p. 715-720.

Research output: Contribution to journalArticle

@article{17d5c175d1384fb1b37ccdf498aed14b,
title = "Empirical study on grouping behavior of individual endothelial cells under shear stress",
abstract = "It has been recognized that the structure and function of the endothelial monolayer is affected by the applied mechanical stress. Microscopic observation of changes in cell morphology during the imposition of shear stress is necessary to identify the specific mechanism which mediates the cellular response to shear stress. In this study we observed the morphology and migration of individual cultured cells with the wall shear stress of 3 and 5 Pa under an optical microscope. We traced the particular group of cells under view, and examined the behavior of these particular cells during the imposition of shear stress. It was concluded that the initial orientation and shape of cells affect the process of receiving the external shear stress stimulus. The behavior of the individual cell depends on the geometry of surrounding cells. Thus, the external shear stress stimulus results in the {"}small grouping behavior{"} , primarily due to the biological and mechanical interaction among cells.",
keywords = "Bio-Fluid Mechanics, Biological Engineering, Biomechanics, Cell Morphology, Endothelial Cell",
author = "Kazuo Tanishita and Kazuhiro Nagayama and Madoka Fujii and Susumu Kudou",
year = "1999",
month = "9",
language = "English",
volume = "42",
pages = "715--720",
journal = "JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing",
issn = "1344-7653",
publisher = "Japan Society of Mechanical Engineers",
number = "3",

}

TY - JOUR

T1 - Empirical study on grouping behavior of individual endothelial cells under shear stress

AU - Tanishita, Kazuo

AU - Nagayama, Kazuhiro

AU - Fujii, Madoka

AU - Kudou, Susumu

PY - 1999/9

Y1 - 1999/9

N2 - It has been recognized that the structure and function of the endothelial monolayer is affected by the applied mechanical stress. Microscopic observation of changes in cell morphology during the imposition of shear stress is necessary to identify the specific mechanism which mediates the cellular response to shear stress. In this study we observed the morphology and migration of individual cultured cells with the wall shear stress of 3 and 5 Pa under an optical microscope. We traced the particular group of cells under view, and examined the behavior of these particular cells during the imposition of shear stress. It was concluded that the initial orientation and shape of cells affect the process of receiving the external shear stress stimulus. The behavior of the individual cell depends on the geometry of surrounding cells. Thus, the external shear stress stimulus results in the "small grouping behavior" , primarily due to the biological and mechanical interaction among cells.

AB - It has been recognized that the structure and function of the endothelial monolayer is affected by the applied mechanical stress. Microscopic observation of changes in cell morphology during the imposition of shear stress is necessary to identify the specific mechanism which mediates the cellular response to shear stress. In this study we observed the morphology and migration of individual cultured cells with the wall shear stress of 3 and 5 Pa under an optical microscope. We traced the particular group of cells under view, and examined the behavior of these particular cells during the imposition of shear stress. It was concluded that the initial orientation and shape of cells affect the process of receiving the external shear stress stimulus. The behavior of the individual cell depends on the geometry of surrounding cells. Thus, the external shear stress stimulus results in the "small grouping behavior" , primarily due to the biological and mechanical interaction among cells.

KW - Bio-Fluid Mechanics

KW - Biological Engineering

KW - Biomechanics

KW - Cell Morphology

KW - Endothelial Cell

UR - http://www.scopus.com/inward/record.url?scp=0033185118&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033185118&partnerID=8YFLogxK

M3 - Article

VL - 42

SP - 715

EP - 720

JO - JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing

JF - JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing

SN - 1344-7653

IS - 3

ER -