Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly(ethylene glycol)-based 4-armed star polymer

Tetsushi Taguchi; Liming Xu; Hisatoshi Kobayashi; Akiyoshi Taniguchi; Kazunori Kataoka; Junzo Tanaka

doi:10.1016/j.biomaterials.2004.04.029

Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly(ethylene glycol)-based 4-armed star polymer

Tetsushi Taguchi^*, Liming Xu, Hisatoshi Kobayashi, Akiyoshi Taniguchi, Kazunori Kataoka, Junzo Tanaka

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

77 Citations (Scopus)

Abstract

An in situ gel system was developed to encapsulate chondrocytes under physiological conditions using an alkali-treated collagen (AlCol) and pentaerythritol poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-PEG) as a crosslinker. AlCol gels were obtained at crosslinker concentrations from 0.1 to 3.0mM. Chondrocytes were encapsulated and dispersed homogeneously in AlCol gels. Results of MTT staining showed that cells survived after encapsulation in AlCol gels. Biochemical analysis demonstrated that DNA content in AlCol gels was constant after 3 weeks. Glycosaminoglycan content and mRNA expression of type II collagen and aggrecan increased with culture time. These results suggest that this in situ gel system is useful for regenerating cartilage in vitro and for minimally invasive therapy for cartilage defects.

Original language	English
Pages (from-to)	1247-1252
Number of pages	6
Journal	Biomaterials
Volume	26
Issue number	11
DOIs	https://doi.org/10.1016/j.biomaterials.2004.04.029
Publication status	Published - 2005 Apr
Externally published	Yes

Keywords

Alkali-treated collagen
Chondrocytes
Polyethylene glycol
Star polymer

ASJC Scopus subject areas

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

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10.1016/j.biomaterials.2004.04.029

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title = "Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly(ethylene glycol)-based 4-armed star polymer",

abstract = "An in situ gel system was developed to encapsulate chondrocytes under physiological conditions using an alkali-treated collagen (AlCol) and pentaerythritol poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-PEG) as a crosslinker. AlCol gels were obtained at crosslinker concentrations from 0.1 to 3.0mM. Chondrocytes were encapsulated and dispersed homogeneously in AlCol gels. Results of MTT staining showed that cells survived after encapsulation in AlCol gels. Biochemical analysis demonstrated that DNA content in AlCol gels was constant after 3 weeks. Glycosaminoglycan content and mRNA expression of type II collagen and aggrecan increased with culture time. These results suggest that this in situ gel system is useful for regenerating cartilage in vitro and for minimally invasive therapy for cartilage defects.",

keywords = "Alkali-treated collagen, Chondrocytes, Polyethylene glycol, Star polymer",

author = "Tetsushi Taguchi and Liming Xu and Hisatoshi Kobayashi and Akiyoshi Taniguchi and Kazunori Kataoka and Junzo Tanaka",

note = "Funding Information: We thank Drs. Y. Mandai and Y. Hiraoka of Nitta Gelatin Co. Ltd., for their invaluable advice and Ms. K. Tateno of Biomaterials Center, National Institute for Materials Science, for technical support. This work was financially supported in part by Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST).",

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doi = "10.1016/j.biomaterials.2004.04.029",

language = "English",

volume = "26",

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T1 - Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly(ethylene glycol)-based 4-armed star polymer

AU - Taguchi, Tetsushi

AU - Xu, Liming

AU - Kobayashi, Hisatoshi

AU - Taniguchi, Akiyoshi

AU - Kataoka, Kazunori

AU - Tanaka, Junzo

N1 - Funding Information: We thank Drs. Y. Mandai and Y. Hiraoka of Nitta Gelatin Co. Ltd., for their invaluable advice and Ms. K. Tateno of Biomaterials Center, National Institute for Materials Science, for technical support. This work was financially supported in part by Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST).

PY - 2005/4

Y1 - 2005/4

N2 - An in situ gel system was developed to encapsulate chondrocytes under physiological conditions using an alkali-treated collagen (AlCol) and pentaerythritol poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-PEG) as a crosslinker. AlCol gels were obtained at crosslinker concentrations from 0.1 to 3.0mM. Chondrocytes were encapsulated and dispersed homogeneously in AlCol gels. Results of MTT staining showed that cells survived after encapsulation in AlCol gels. Biochemical analysis demonstrated that DNA content in AlCol gels was constant after 3 weeks. Glycosaminoglycan content and mRNA expression of type II collagen and aggrecan increased with culture time. These results suggest that this in situ gel system is useful for regenerating cartilage in vitro and for minimally invasive therapy for cartilage defects.

AB - An in situ gel system was developed to encapsulate chondrocytes under physiological conditions using an alkali-treated collagen (AlCol) and pentaerythritol poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-PEG) as a crosslinker. AlCol gels were obtained at crosslinker concentrations from 0.1 to 3.0mM. Chondrocytes were encapsulated and dispersed homogeneously in AlCol gels. Results of MTT staining showed that cells survived after encapsulation in AlCol gels. Biochemical analysis demonstrated that DNA content in AlCol gels was constant after 3 weeks. Glycosaminoglycan content and mRNA expression of type II collagen and aggrecan increased with culture time. These results suggest that this in situ gel system is useful for regenerating cartilage in vitro and for minimally invasive therapy for cartilage defects.

KW - Alkali-treated collagen

KW - Chondrocytes

KW - Polyethylene glycol

KW - Star polymer

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Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly(ethylene glycol)-based 4-armed star polymer

Abstract

Keywords

ASJC Scopus subject areas

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