Encapsulation of Hb into unsaturated lipid vesicles and γ‐ray polymerization

Hiromi Sakai, Shinji Takeoka, Hiroaki Yokohama, Hiroyuki Nishide, Eishun Tsuchida

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A carbonyl hemoglobin (HbCO) solution was stirred with a mixed powder of polymerizable 1,2‐bis(2,4‐octadecadienoyl)‐sn‐glycero‐3‐phosphocholine (DODPC), cholesterol and stearic acid (7/7/2 by mol). The mixture was extruded through polycarbonate membrane filters (final pore size = 0.2 μm Ø). The average diameter of the resulting vesicles was 203 ± 39 nm. The [Hb]/[Lipid] ratio (the weight ratio of Hb in vesicle to lipid) increased with the Hb concentration, and decreased with the NaCl concentration. A maximum [Hb]/[lipid] ratio was observed at pH 6.9, which was the same as the isoelectric point of Hb. The vesicles were stabilized by γ‐irradiation (60Co) because the bilayer lipids bound each other to yield polyphospholipids. Denaturation of Hb by γ‐irradiation was not detected. These polyphospholipid vesicles encapsulating Hb were stable even against the freeze–thaw cycles and the freeze‐drying procedure.

Original languageEnglish
Pages (from-to)389-394
Number of pages6
JournalPolymers for Advanced Technologies
Volume3
Issue number7
DOIs
Publication statusPublished - 1992

Fingerprint

Encapsulation
Lipids
polycarbonate
Polymerization
Irradiation
Carboxyhemoglobin
Denaturation
Lipid bilayers
Stearic acid
Hemoglobin
Cholesterol
Polycarbonates
Powders
Pore size
Membranes

Keywords

  • Blood substitute
  • Encapsulation
  • Hemoglobin
  • Lipid vesicle
  • γ‐Ray polymerization

ASJC Scopus subject areas

  • Polymers and Plastics

Cite this

Encapsulation of Hb into unsaturated lipid vesicles and γ‐ray polymerization. / Sakai, Hiromi; Takeoka, Shinji; Yokohama, Hiroaki; Nishide, Hiroyuki; Tsuchida, Eishun.

In: Polymers for Advanced Technologies, Vol. 3, No. 7, 1992, p. 389-394.

Research output: Contribution to journalArticle

@article{c36aa6eaea214b68bb1afdc1fa0ad986,
title = "Encapsulation of Hb into unsaturated lipid vesicles and γ‐ray polymerization",
abstract = "A carbonyl hemoglobin (HbCO) solution was stirred with a mixed powder of polymerizable 1,2‐bis(2,4‐octadecadienoyl)‐sn‐glycero‐3‐phosphocholine (DODPC), cholesterol and stearic acid (7/7/2 by mol). The mixture was extruded through polycarbonate membrane filters (final pore size = 0.2 μm {\O}). The average diameter of the resulting vesicles was 203 ± 39 nm. The [Hb]/[Lipid] ratio (the weight ratio of Hb in vesicle to lipid) increased with the Hb concentration, and decreased with the NaCl concentration. A maximum [Hb]/[lipid] ratio was observed at pH 6.9, which was the same as the isoelectric point of Hb. The vesicles were stabilized by γ‐irradiation (60Co) because the bilayer lipids bound each other to yield polyphospholipids. Denaturation of Hb by γ‐irradiation was not detected. These polyphospholipid vesicles encapsulating Hb were stable even against the freeze–thaw cycles and the freeze‐drying procedure.",
keywords = "Blood substitute, Encapsulation, Hemoglobin, Lipid vesicle, γ‐Ray polymerization",
author = "Hiromi Sakai and Shinji Takeoka and Hiroaki Yokohama and Hiroyuki Nishide and Eishun Tsuchida",
year = "1992",
doi = "10.1002/pat.1992.220030704",
language = "English",
volume = "3",
pages = "389--394",
journal = "Polymers for Advanced Technologies",
issn = "1042-7147",
publisher = "John Wiley and Sons Ltd",
number = "7",

}

TY - JOUR

T1 - Encapsulation of Hb into unsaturated lipid vesicles and γ‐ray polymerization

AU - Sakai, Hiromi

AU - Takeoka, Shinji

AU - Yokohama, Hiroaki

AU - Nishide, Hiroyuki

AU - Tsuchida, Eishun

PY - 1992

Y1 - 1992

N2 - A carbonyl hemoglobin (HbCO) solution was stirred with a mixed powder of polymerizable 1,2‐bis(2,4‐octadecadienoyl)‐sn‐glycero‐3‐phosphocholine (DODPC), cholesterol and stearic acid (7/7/2 by mol). The mixture was extruded through polycarbonate membrane filters (final pore size = 0.2 μm Ø). The average diameter of the resulting vesicles was 203 ± 39 nm. The [Hb]/[Lipid] ratio (the weight ratio of Hb in vesicle to lipid) increased with the Hb concentration, and decreased with the NaCl concentration. A maximum [Hb]/[lipid] ratio was observed at pH 6.9, which was the same as the isoelectric point of Hb. The vesicles were stabilized by γ‐irradiation (60Co) because the bilayer lipids bound each other to yield polyphospholipids. Denaturation of Hb by γ‐irradiation was not detected. These polyphospholipid vesicles encapsulating Hb were stable even against the freeze–thaw cycles and the freeze‐drying procedure.

AB - A carbonyl hemoglobin (HbCO) solution was stirred with a mixed powder of polymerizable 1,2‐bis(2,4‐octadecadienoyl)‐sn‐glycero‐3‐phosphocholine (DODPC), cholesterol and stearic acid (7/7/2 by mol). The mixture was extruded through polycarbonate membrane filters (final pore size = 0.2 μm Ø). The average diameter of the resulting vesicles was 203 ± 39 nm. The [Hb]/[Lipid] ratio (the weight ratio of Hb in vesicle to lipid) increased with the Hb concentration, and decreased with the NaCl concentration. A maximum [Hb]/[lipid] ratio was observed at pH 6.9, which was the same as the isoelectric point of Hb. The vesicles were stabilized by γ‐irradiation (60Co) because the bilayer lipids bound each other to yield polyphospholipids. Denaturation of Hb by γ‐irradiation was not detected. These polyphospholipid vesicles encapsulating Hb were stable even against the freeze–thaw cycles and the freeze‐drying procedure.

KW - Blood substitute

KW - Encapsulation

KW - Hemoglobin

KW - Lipid vesicle

KW - γ‐Ray polymerization

UR - http://www.scopus.com/inward/record.url?scp=84994946168&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994946168&partnerID=8YFLogxK

U2 - 10.1002/pat.1992.220030704

DO - 10.1002/pat.1992.220030704

M3 - Article

VL - 3

SP - 389

EP - 394

JO - Polymers for Advanced Technologies

JF - Polymers for Advanced Technologies

SN - 1042-7147

IS - 7

ER -