Endogenous carbon monoxide suppression stimulates bile acid-dependent biliary transport in perfused rat liver

Tsuyoshi Sano, Masaya Shiomi, Yoshiyuki Wakabayashi, Yuichi Shinoda, Nobuhito Goda, Tokio Yamaguchi, Yuji Nimura, Yuzuru Ishimura, Makoto Suematsu

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

This study aimed to investigate whether carbon monoxide (CO), a product of heme oxygenase that degrades protoheme IX, serves as an endogenous modulator for biliary transport. To that end, effects of zinc protoporphyrin IX (ZnPP), a hems oxygenase inhibitor, on the biliary transport were tested in perfused rat liver. Perfusion of 1 μM ZnPP abolished detectable levels of CO in the venous perfusate and increased bile acid-dependent bile output accompanying an increased secretion of bile salts. The ZnPP-induced choleresis coincided with a reduction of tissue guanosine 3',5'-cyclic monophosphate (cGMP) levels and a decrease in vascular conductance. On administration of 2.5 μM CO, ZnPP-elicited choleresis, decreases in vascular conductance, and cGMP levels were all attenuated. Treatment with 1 μM 8- bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP) partly attenuated the ZnPP-induced choleresis in concert with repression of vascular conductance. Furthermore, treatment of the liver with methylene blue, a guanylate cyclase inhibitor, evoked a choleresis similar to that induced by ZnPP. Thus endogenous CO suppression stimulates the biliary transport in part through a cGMP-dependent mechanism.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume272
Issue number5 35-5
Publication statusPublished - 1997 May
Externally publishedYes

Fingerprint

Carbon Monoxide
Bile Acids and Salts
Liver
Blood Vessels
Heme Oxygenase (Decyclizing)
Oxygenases
Guanylate Cyclase
Methylene Blue
Cyclic GMP
Heme
Bile
zinc protoporphyrin
Perfusion

Keywords

  • Adenosine 5'-triphosphate
  • Bile transport
  • Guanosine 3',5'-cyclic monophosphate
  • Heme oxygenase
  • Nitric oxide

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology
  • Physiology (medical)

Cite this

Endogenous carbon monoxide suppression stimulates bile acid-dependent biliary transport in perfused rat liver. / Sano, Tsuyoshi; Shiomi, Masaya; Wakabayashi, Yoshiyuki; Shinoda, Yuichi; Goda, Nobuhito; Yamaguchi, Tokio; Nimura, Yuji; Ishimura, Yuzuru; Suematsu, Makoto.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 272, No. 5 35-5, 05.1997.

Research output: Contribution to journalArticle

Sano, T, Shiomi, M, Wakabayashi, Y, Shinoda, Y, Goda, N, Yamaguchi, T, Nimura, Y, Ishimura, Y & Suematsu, M 1997, 'Endogenous carbon monoxide suppression stimulates bile acid-dependent biliary transport in perfused rat liver', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 272, no. 5 35-5.
Sano, Tsuyoshi ; Shiomi, Masaya ; Wakabayashi, Yoshiyuki ; Shinoda, Yuichi ; Goda, Nobuhito ; Yamaguchi, Tokio ; Nimura, Yuji ; Ishimura, Yuzuru ; Suematsu, Makoto. / Endogenous carbon monoxide suppression stimulates bile acid-dependent biliary transport in perfused rat liver. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 1997 ; Vol. 272, No. 5 35-5.
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