Endothelial cell marker gene expression on double-layered co-culture utilizing thermo-responsive culture dish

Y. Shirai, Y. Kurosawa, T. Okano, A. Taniguchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Endothelial cell marker gene expression on the reconstruction of double-layered co-culture endothelial cells was investigated using thermo-responsive culture dish. Human umbilical vein endothelial cells (HUVEC) at passage 3 were cultured in modified EGM-2 for the experiments. The gene expression levels of PECAM-1, ICAM-1 and Factor VIII on double-layered co-culture endothelial cells were increased after 2 weeks compared with monolayer culture. The results suggests that hepatocyte functions and endothelial cell functions are maintained and liver parenchyma during double-layered co-culture.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Number of pages1
Publication statusPublished - 2004 Dec 1
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: 2004 May 172004 May 21

Publication series

NameTransactions - 7th World Biomaterials Congress

Other

OtherTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period04/5/1704/5/21

ASJC Scopus subject areas

  • Engineering(all)

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