Endothelial cell marker gene expression on double-layered co-culture utilizing thermo-responsive culture dish

Y. Shirai, Y. Kurosawa, T. Okano, Akiyoshi Taniguchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Endothelial cell marker gene expression on the reconstruction of double-layered co-culture endothelial cells was investigated using thermo-responsive culture dish. Human umbilical vein endothelial cells (HUVEC) at passage 3 were cultured in modified EGM-2 for the experiments. The gene expression levels of PECAM-1, ICAM-1 and Factor VIII on double-layered co-culture endothelial cells were increased after 2 weeks compared with monolayer culture. The results suggests that hepatocyte functions and endothelial cell functions are maintained and liver parenchyma during double-layered co-culture.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Pages315
Number of pages1
Publication statusPublished - 2004
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: 2004 May 172004 May 21

Other

OtherTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period04/5/1704/5/21

Fingerprint

Endothelial cells
Cell culture
Gene expression
Liver
Monolayers
Experiments

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Shirai, Y., Kurosawa, Y., Okano, T., & Taniguchi, A. (2004). Endothelial cell marker gene expression on double-layered co-culture utilizing thermo-responsive culture dish. In Transactions - 7th World Biomaterials Congress (pp. 315)

Endothelial cell marker gene expression on double-layered co-culture utilizing thermo-responsive culture dish. / Shirai, Y.; Kurosawa, Y.; Okano, T.; Taniguchi, Akiyoshi.

Transactions - 7th World Biomaterials Congress. 2004. p. 315.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shirai, Y, Kurosawa, Y, Okano, T & Taniguchi, A 2004, Endothelial cell marker gene expression on double-layered co-culture utilizing thermo-responsive culture dish. in Transactions - 7th World Biomaterials Congress. pp. 315, Transactions - 7th World Biomaterials Congress, Sydney, Australia, 04/5/17.
Shirai Y, Kurosawa Y, Okano T, Taniguchi A. Endothelial cell marker gene expression on double-layered co-culture utilizing thermo-responsive culture dish. In Transactions - 7th World Biomaterials Congress. 2004. p. 315
Shirai, Y. ; Kurosawa, Y. ; Okano, T. ; Taniguchi, Akiyoshi. / Endothelial cell marker gene expression on double-layered co-culture utilizing thermo-responsive culture dish. Transactions - 7th World Biomaterials Congress. 2004. pp. 315
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