Endothelial progenitor cells promote directional three-dimensional endothelial network formation by secreting vascular endothelial growth factor

Yoshinori Abe, Yoshiyuki Ozaki, Junichi Kasuya, Kimiko Yamamoto, Joji Ando, Ryo Sudo, Mariko Ikeda, Kazuo Tanishita

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Endothelial progenitor cell (EPC) transplantation induces the formation of new blood-vessel networks to supply nutrients and oxygen, and is feasible for the treatment of ischemia and cardiovascular diseases. However, the role of EPCs as a source of proangiogenic cytokines and consequent generators of an extracellular growth factor microenvironment in three-dimensional (3D) microvessel formation is not fully understood. We focused on the contribution of EPCs as a source of proangiogenic cytokines on 3D microvessel formation using an in vitro 3D network model. To create a 3D network model, EPCs isolated from rat bone marrow were sandwiched with double layers of collagen gel. Endothelial cells (ECs) were then cultured on top of the upper collagen gel layer. Quantitative analyses of EC network formation revealed that the length, number, and depth of the EC networks were significantly enhanced in a 3D model with ECs and EPCs compared to an EC monoculture. In addition, conditioned medium (CM) from the 3D model with ECs and EPCs promoted network formation compared to CM from an EC monoculture. We also confirmed that EPCs secreted vascular endothelial growth factor (VEGF). However, networks cultured with the CM were shallow and did not penetrate the collagen gel in great depth. Therefore, we conclude that EPCs contribute to 3D network formation at least through indirect incorporation by generating a local VEGF gradient. These results suggest that the location of EPCs is important for controlling directional 3D network formation in the field of tissue engineering.

Original languageEnglish
Article numbere82085
JournalPLoS One
Volume8
Issue number12
DOIs
Publication statusPublished - 2013 Dec 3

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vascular endothelial growth factors
Endothelial cells
Vascular Endothelial Growth Factor A
endothelial cells
stem cells
Endothelial Cells
Conditioned Culture Medium
collagen
Collagen
Gels
gels
Microvessels
cytokines
Cytokines
tissue engineering
cell transplantation
Endothelial Progenitor Cells
erucylphosphocholine
generators (equipment)
Cell Transplantation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Endothelial progenitor cells promote directional three-dimensional endothelial network formation by secreting vascular endothelial growth factor. / Abe, Yoshinori; Ozaki, Yoshiyuki; Kasuya, Junichi; Yamamoto, Kimiko; Ando, Joji; Sudo, Ryo; Ikeda, Mariko; Tanishita, Kazuo.

In: PLoS One, Vol. 8, No. 12, e82085, 03.12.2013.

Research output: Contribution to journalArticle

Abe, Yoshinori ; Ozaki, Yoshiyuki ; Kasuya, Junichi ; Yamamoto, Kimiko ; Ando, Joji ; Sudo, Ryo ; Ikeda, Mariko ; Tanishita, Kazuo. / Endothelial progenitor cells promote directional three-dimensional endothelial network formation by secreting vascular endothelial growth factor. In: PLoS One. 2013 ; Vol. 8, No. 12.
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