Endurance exercise training enhances local sex steroidogenesis in skeletal muscle

Katsuji Aizawa, Motoyuki Iemitsu, Seiji Maeda, Noboru Mesaki, Takashi Ushida, Takayuki Akimoto

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Purpose: Endurance training improves skeletal muscular function including energy metabolism and structure. Sex steroid hormones partly contribute to the exercise-induced muscular adaptations. Recently, we demonstrated that skeletal muscle contains steroidogenic converting enzymes to synthesize sex steroid hormones and an acute endurance exercise activates local steroidogenesis in skeletal muscle. However, whether chronic endurance training leads to enhanced steroidogenesis in skeletal muscle is unknown. Here, we examined changes in steroidogenic enzymes and sex steroid hormones in the skeletal muscle after chronic endurance exercise training. Methods: Eleven male rats were divided into two groups: sedentary (n = 6) and trained (n = 5). Endurance training was performed on a treadmill (30 m•min, 30 min) for 5 d•wk for 12 wk. The posttraining harvesting was performed 48 h after the last exercise training. Results: The mRNA expressions of 3β-HSD, aromatase cytochrome P450, and 5α-reductase in the skeletal muscle of trained rats were significantly higher than those of sedentary rats (P < 0.05). The protein expressions of aromatase cytochrome P450 and 5α-reductase in the skeletal muscle of trained rats were also significantly higher than those of sedentary rats (P < 0.05). The muscular dihydrotestosterone (DHT) concentrations in the skeletal muscle of trained rats were significantly higher than those of sedentary rats (P < 0.01), but there was no change in dehydroepiandrosterone, total testosterone, free testosterone, and estradiol. Furthermore, muscle weight corrected for body weight of trained rats was moderately correlated with the level of muscular DHT concentration in trained rats (r = 0.41, P < 0.05). Conclusions: Endurance exercise training enhances the muscular DHT concentration through 5α-reductase in the skeletal muscle of rats, suggesting that local bioactive androgen metabolism may participate in exercise training-induced skeletal muscular adaptation.

Original languageEnglish
Pages (from-to)2072-2080
Number of pages9
JournalMedicine and Science in Sports and Exercise
Volume43
Issue number11
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Skeletal Muscle
Exercise
Dihydrotestosterone
Gonadal Steroid Hormones
NADPH-Ferrihemoprotein Reductase
Aromatase
Testosterone
Dehydroepiandrosterone
Enzymes
Energy Metabolism
Androgens
Estradiol
Oxidoreductases
Body Weight
Weights and Measures
Muscles
Messenger RNA

Keywords

  • Endurance training
  • sex steroid hormone
  • skeletal muscle
  • steroidogenic enzyme

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Endurance exercise training enhances local sex steroidogenesis in skeletal muscle. / Aizawa, Katsuji; Iemitsu, Motoyuki; Maeda, Seiji; Mesaki, Noboru; Ushida, Takashi; Akimoto, Takayuki.

In: Medicine and Science in Sports and Exercise, Vol. 43, No. 11, 2011, p. 2072-2080.

Research output: Contribution to journalArticle

Aizawa, Katsuji ; Iemitsu, Motoyuki ; Maeda, Seiji ; Mesaki, Noboru ; Ushida, Takashi ; Akimoto, Takayuki. / Endurance exercise training enhances local sex steroidogenesis in skeletal muscle. In: Medicine and Science in Sports and Exercise. 2011 ; Vol. 43, No. 11. pp. 2072-2080.
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