Enhanced erythropoiesis in mice exposed to low environmental temperature

Shun Maekawa, Hitomi Iemura, Takashi Kato

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Hematopoietic responses to environmental factors are not fully characterized. Polycythemia has been reported during exposure to low temperatures in ectothermic animals. The relationship between the causes of polycythemia and erythropoiesis during low temperature exposure is not fully understood. In this study, we exposed C57BL/6 mice to 5°C and monitored the blood cell counts and erythropoiesis. The hematocrit level increased from 45.6 to 52.2% after 14 days. Likewise, the hemoglobin concentration, initially 15.1 g dl-1, rose to 16.0 g dl-1. The reticulocyte production index significantly increased from 4 to 8% after 7 days. We examined the anatomy and cell composition of the spleens of the mice. On day5, the spleens were ̃6 mg g-1 of body mass, which was twofold greater than the spleens on day0. Flow cytometry showed fourfold more proerythroblasts on day5, compared with day0. Additionally, the number of late-stage mature erythroblasts increased on day14. Erythropoietin mRNA levels increased in the kidneys, and hypoxia-inducible genes were enhanced in the kidney. Our findings indicated that low ambient temperature is a novel erythropoietic stress, which induces polycythemia by enhanced erythropoiesis.

Original languageEnglish
Pages (from-to)901-908
Number of pages8
JournalJournal of Experimental Biology
Volume216
Issue number5
DOIs
Publication statusPublished - 2013 Mar

Keywords

  • Erythropoiesis
  • Low temperature
  • Mouse
  • Polycythemia

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Molecular Biology
  • Insect Science

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