Enhanced erythropoiesis in mice exposed to low environmental temperature

Shun Maekawa, Hitomi Iemura, Takashi Kato

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Hematopoietic responses to environmental factors are not fully characterized. Polycythemia has been reported during exposure to low temperatures in ectothermic animals. The relationship between the causes of polycythemia and erythropoiesis during low temperature exposure is not fully understood. In this study, we exposed C57BL/6 mice to 5°C and monitored the blood cell counts and erythropoiesis. The hematocrit level increased from 45.6 to 52.2% after 14 days. Likewise, the hemoglobin concentration, initially 15.1 g dl-1, rose to 16.0 g dl-1. The reticulocyte production index significantly increased from 4 to 8% after 7 days. We examined the anatomy and cell composition of the spleens of the mice. On day5, the spleens were ̃6 mg g-1 of body mass, which was twofold greater than the spleens on day0. Flow cytometry showed fourfold more proerythroblasts on day5, compared with day0. Additionally, the number of late-stage mature erythroblasts increased on day14. Erythropoietin mRNA levels increased in the kidneys, and hypoxia-inducible genes were enhanced in the kidney. Our findings indicated that low ambient temperature is a novel erythropoietic stress, which induces polycythemia by enhanced erythropoiesis.

    Original languageEnglish
    Pages (from-to)901-908
    Number of pages8
    JournalJournal of Experimental Biology
    Volume216
    Issue number5
    DOIs
    Publication statusPublished - 2013 Mar

    Fingerprint

    polycythemia
    erythropoiesis
    Polycythemia
    Erythropoiesis
    Erythroblasts
    ambient temperature
    spleen
    Spleen
    Temperature
    mice
    flow cytometry
    hemoglobin
    hypoxia
    kidneys
    erythroblasts
    anatomy
    body mass
    Kidney
    erythropoietin
    blood cell counts

    Keywords

    • Erythropoiesis
    • Low temperature
    • Mouse
    • Polycythemia

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • Ecology, Evolution, Behavior and Systematics
    • Molecular Biology
    • Physiology
    • Insect Science
    • Aquatic Science

    Cite this

    Enhanced erythropoiesis in mice exposed to low environmental temperature. / Maekawa, Shun; Iemura, Hitomi; Kato, Takashi.

    In: Journal of Experimental Biology, Vol. 216, No. 5, 03.2013, p. 901-908.

    Research output: Contribution to journalArticle

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