Enhanced extracellular production of heterologous proteins in bacillus subtilis by deleting the c-terminal region of the SecA secretory machinery

Hiroshi Kakeshtia, Yasushi Kageyama, Katsutoshi Ara, Katsuya Ozaki, Kouji Nakamura

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In this study, we examined the effects of modifying the C-terminal region of the SecA protein on the production of heterologous proteins in Bacillus subtilis. SecA was selected as a candidate among the components of the Sec system due to its ability to interact directly with both the precursors and membrane translocases. A phylogenetic comparison demonstrated that the C-terminal region is not well conserved among eubacterial SecA proteins. The deletion of the 61 amino acids at the C-terminal region led to an 83% increase in extracellular alkaliphilic Bacillus sp. thermostable alkaline cellulase (Egl-237) activity. Moreover, the productivity of human interferon α (hIFN-α2b) was increased by 2.2-fold compared to the wild-type SecA, by deletion of these 61 amino acids. We indicated that the deletion of the C-terminal domain (CTD) of SecA enhanced the secretion of two different heterologous protein, Egl-237 and hIFN-α2b. This study provides a useful method to enhance the extracellular production of heterologous proteins in B. subtilis.

Original languageEnglish
Pages (from-to)250-257
Number of pages8
JournalMolecular Biotechnology
Volume46
Issue number3
DOIs
Publication statusPublished - 2010 Nov
Externally publishedYes

Keywords

  • Alkaliphilic Bacillus sp. thermostable alkaline cellulase
  • Bacillus subtilis
  • Heterologous proteins
  • Human interferon α
  • Protein secretion
  • SecA

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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