Enhanced germination of artificial seeds by marine cynobacterial extract

Hitoshi Wake, Akinobu Akasaka, Hironori Umetsu, Yoshihiro Ozeki, Koichiro Shimomura, Tadashi Matsunaga

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have developed an improved artificial seed system by using a hot-water extract from a marine cyanobacterium, Synechococcus sp. NKBG 042902. Carrot somatic embryos (Daucus carota L.) were divided into two size categories (> 800 μm and 425-800 μm). High frequency germination (91%) was obtained using the large somatic embryos encapsulated in calcium alginate gel containing 400 mg 1-1 of extract. This compares to 35% without addition of the extract. A non-dialysate fraction of the extract showed strong germination-promoting activity compared with a dialysate fraction. The germination frequency of artificial seeds containing 100 mg 1-1 of non-dialysate fraction was more than 90%. Almost all germinating artificial seeds developed into plantlets within 4 days. We also achieved high frequency germination (60%) of artificial seeds encapsulating small somatic embryos (425-800 μm) that contained 100 mg 1-1 of non-dialysate (control 9%). Although the small somatic embryos showed a lower germination frequency than the large embryos, the plantlet development process in these seeds was far more vigorous. Such a high germination frequency has not previously been reported for a carrot artificial seed system.

Original languageEnglish
Pages (from-to)684-688
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume36
Issue number5
DOIs
Publication statusPublished - 1992 Feb
Externally publishedYes

Fingerprint

Germination
Seeds
Daucus carota
Embryonic Structures
Synechococcus
Dialysis Solutions
Cyanobacteria
Embryonic Development
Gels
Water

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Wake, H., Akasaka, A., Umetsu, H., Ozeki, Y., Shimomura, K., & Matsunaga, T. (1992). Enhanced germination of artificial seeds by marine cynobacterial extract. Applied Microbiology and Biotechnology, 36(5), 684-688. https://doi.org/10.1007/BF00183250

Enhanced germination of artificial seeds by marine cynobacterial extract. / Wake, Hitoshi; Akasaka, Akinobu; Umetsu, Hironori; Ozeki, Yoshihiro; Shimomura, Koichiro; Matsunaga, Tadashi.

In: Applied Microbiology and Biotechnology, Vol. 36, No. 5, 02.1992, p. 684-688.

Research output: Contribution to journalArticle

Wake, H, Akasaka, A, Umetsu, H, Ozeki, Y, Shimomura, K & Matsunaga, T 1992, 'Enhanced germination of artificial seeds by marine cynobacterial extract', Applied Microbiology and Biotechnology, vol. 36, no. 5, pp. 684-688. https://doi.org/10.1007/BF00183250
Wake, Hitoshi ; Akasaka, Akinobu ; Umetsu, Hironori ; Ozeki, Yoshihiro ; Shimomura, Koichiro ; Matsunaga, Tadashi. / Enhanced germination of artificial seeds by marine cynobacterial extract. In: Applied Microbiology and Biotechnology. 1992 ; Vol. 36, No. 5. pp. 684-688.
@article{9a5b3a9a47364e0c9b3c4acbf3e47942,
title = "Enhanced germination of artificial seeds by marine cynobacterial extract",
abstract = "We have developed an improved artificial seed system by using a hot-water extract from a marine cyanobacterium, Synechococcus sp. NKBG 042902. Carrot somatic embryos (Daucus carota L.) were divided into two size categories (> 800 μm and 425-800 μm). High frequency germination (91{\%}) was obtained using the large somatic embryos encapsulated in calcium alginate gel containing 400 mg 1-1 of extract. This compares to 35{\%} without addition of the extract. A non-dialysate fraction of the extract showed strong germination-promoting activity compared with a dialysate fraction. The germination frequency of artificial seeds containing 100 mg 1-1 of non-dialysate fraction was more than 90{\%}. Almost all germinating artificial seeds developed into plantlets within 4 days. We also achieved high frequency germination (60{\%}) of artificial seeds encapsulating small somatic embryos (425-800 μm) that contained 100 mg 1-1 of non-dialysate (control 9{\%}). Although the small somatic embryos showed a lower germination frequency than the large embryos, the plantlet development process in these seeds was far more vigorous. Such a high germination frequency has not previously been reported for a carrot artificial seed system.",
author = "Hitoshi Wake and Akinobu Akasaka and Hironori Umetsu and Yoshihiro Ozeki and Koichiro Shimomura and Tadashi Matsunaga",
year = "1992",
month = "2",
doi = "10.1007/BF00183250",
language = "English",
volume = "36",
pages = "684--688",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "5",

}

TY - JOUR

T1 - Enhanced germination of artificial seeds by marine cynobacterial extract

AU - Wake, Hitoshi

AU - Akasaka, Akinobu

AU - Umetsu, Hironori

AU - Ozeki, Yoshihiro

AU - Shimomura, Koichiro

AU - Matsunaga, Tadashi

PY - 1992/2

Y1 - 1992/2

N2 - We have developed an improved artificial seed system by using a hot-water extract from a marine cyanobacterium, Synechococcus sp. NKBG 042902. Carrot somatic embryos (Daucus carota L.) were divided into two size categories (> 800 μm and 425-800 μm). High frequency germination (91%) was obtained using the large somatic embryos encapsulated in calcium alginate gel containing 400 mg 1-1 of extract. This compares to 35% without addition of the extract. A non-dialysate fraction of the extract showed strong germination-promoting activity compared with a dialysate fraction. The germination frequency of artificial seeds containing 100 mg 1-1 of non-dialysate fraction was more than 90%. Almost all germinating artificial seeds developed into plantlets within 4 days. We also achieved high frequency germination (60%) of artificial seeds encapsulating small somatic embryos (425-800 μm) that contained 100 mg 1-1 of non-dialysate (control 9%). Although the small somatic embryos showed a lower germination frequency than the large embryos, the plantlet development process in these seeds was far more vigorous. Such a high germination frequency has not previously been reported for a carrot artificial seed system.

AB - We have developed an improved artificial seed system by using a hot-water extract from a marine cyanobacterium, Synechococcus sp. NKBG 042902. Carrot somatic embryos (Daucus carota L.) were divided into two size categories (> 800 μm and 425-800 μm). High frequency germination (91%) was obtained using the large somatic embryos encapsulated in calcium alginate gel containing 400 mg 1-1 of extract. This compares to 35% without addition of the extract. A non-dialysate fraction of the extract showed strong germination-promoting activity compared with a dialysate fraction. The germination frequency of artificial seeds containing 100 mg 1-1 of non-dialysate fraction was more than 90%. Almost all germinating artificial seeds developed into plantlets within 4 days. We also achieved high frequency germination (60%) of artificial seeds encapsulating small somatic embryos (425-800 μm) that contained 100 mg 1-1 of non-dialysate (control 9%). Although the small somatic embryos showed a lower germination frequency than the large embryos, the plantlet development process in these seeds was far more vigorous. Such a high germination frequency has not previously been reported for a carrot artificial seed system.

UR - http://www.scopus.com/inward/record.url?scp=0026564573&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026564573&partnerID=8YFLogxK

U2 - 10.1007/BF00183250

DO - 10.1007/BF00183250

M3 - Article

AN - SCOPUS:0026564573

VL - 36

SP - 684

EP - 688

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 5

ER -