Enhanced Release of Chitosanase from Streptomyces griseus through Direct Interaction of Liposome with Cell Membrane under Heat Stress

Kien Xuan Ngo, Hiroshi Umakoshi, Toshinori Shimanouchi, Huong Thi Bui, Ryoichi Kuboi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A direct interaction of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes with membrane of Streptomyces griseus cell under the heat stress at 41°C increased the chitosanase production and release to 2.2 times higher than that at 37°C without the POPC liposomes. Amount of chitosanase released across the lipid mimicking cell membrane (LMCM) liposome under a heat at 41°C in the presence of POPC liposomes was 17% of initially-entrapped chitosanase while it was only 1% in the absence of POPC liposomes, even under a heat stress at 41°C, clearly showing the importance of the direct interaction between membrane and membrane.

Original languageEnglish
Pages (from-to)602-605
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume106
Issue number6
DOIs
Publication statusPublished - 2008 Dec
Externally publishedYes

Fingerprint

chitosanase
Streptomyces griseus
Liposomes
Cell membranes
Hot Temperature
Cell Membrane
Membranes
Membrane Lipids
1-palmitoyl-2-oleoylphosphatidylcholine

Keywords

  • chitosanase
  • heat stress
  • liposomes
  • membrane interaction
  • Streptomyces griseus

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Enhanced Release of Chitosanase from Streptomyces griseus through Direct Interaction of Liposome with Cell Membrane under Heat Stress. / Ngo, Kien Xuan; Umakoshi, Hiroshi; Shimanouchi, Toshinori; Bui, Huong Thi; Kuboi, Ryoichi.

In: Journal of Bioscience and Bioengineering, Vol. 106, No. 6, 12.2008, p. 602-605.

Research output: Contribution to journalArticle

Ngo, Kien Xuan ; Umakoshi, Hiroshi ; Shimanouchi, Toshinori ; Bui, Huong Thi ; Kuboi, Ryoichi. / Enhanced Release of Chitosanase from Streptomyces griseus through Direct Interaction of Liposome with Cell Membrane under Heat Stress. In: Journal of Bioscience and Bioengineering. 2008 ; Vol. 106, No. 6. pp. 602-605.
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