Enhanced synthesis of 5-Hydroxy-L-tryptophan through tetrahydropterin regeneration

Ryotaro Hara, Kuniki Kino

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    5-Hydroxy-L-tryptophan (5-HTP) is a naturally occurring aromatic amino acid present in the seeds of the African plant Griffonia simplicifolia. Although 5-HTP has therapeutic effects in various symptoms, efficient method of producing 5-HTP has not been established. In this study, we developed a novel cofactor regeneration process to achieve enhanced synthesis of 5-HTP by using modified L-phenylalanine 4-hydroxylase of Chromobacterium violaceum. For the synthesis of 5-HTP using Escherichia coli whole cell bioconversion, L-tryptophan and 5-HTP degradation by E. coli endogenous catabolic enzymes should be considered. The tryptophanase gene was disrupted using the λ red recombination system, since tryptophanase is postulated as an initial enzyme for the degradation of L-tryptophan and 5-HTP in E. coli. For regeneration of the cofactor pterin, we screened and investigated several key enzymes, including dihydropteridine reductase from E. coli, glucose dehydrogenase from Bacillus subtilis, and pterin-4α-carbinolamine dehydratase from Pseudomonas syringae. Genes encoding these three enzymes were overexpressed in an E. coli tryptophanase-deficient host, resulting in the synthesis of 0.74 mM 5-HTP in the presence of 0.1 mM pterin and the synthesis of 0.07 mM 5-HTP in the absence of regeneration of pterin. These results clearly indicated the successful regeneration of pterin. Following optimization of the reaction conditions, 2.5 mM 5-HTP was synthesized with cofactor regeneration, while 0.8 mM 5-HTP was recovered without cofactor regeneration under the same reaction conditions, suggesting that the principle described here provides a new method for cofactor regeneration.

    Original languageEnglish
    Pages (from-to)1-7
    Number of pages7
    JournalAMB Express
    Volume3
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    5-Hydroxytryptophan
    Tryptophan
    Regeneration
    Pterins
    Tryptophanase
    Escherichia coli
    Enzymes
    tetrahydropterin
    Dihydropteridine Reductase
    Griffonia
    Chromobacterium
    Glucose 1-Dehydrogenase
    Phenylalanine Hydroxylase
    Hydro-Lyases
    Pseudomonas syringae
    Aromatic Amino Acids
    Therapeutic Uses
    Bacillus subtilis
    Phenylalanine
    Genetic Recombination

    Keywords

    • 5-hydroxy-L-tryptophan
    • Cofactor regeneration
    • L-phenylalanine 4-hydroxylase
    • NAD(P)H
    • Tetrahydropterin

    ASJC Scopus subject areas

    • Biophysics
    • Applied Microbiology and Biotechnology

    Cite this

    Enhanced synthesis of 5-Hydroxy-L-tryptophan through tetrahydropterin regeneration. / Hara, Ryotaro; Kino, Kuniki.

    In: AMB Express, Vol. 3, 2013, p. 1-7.

    Research output: Contribution to journalArticle

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