Enhancement and repression of cyanide-insensitive respiration in Aspergillus niger

Kohtaro Kirimura, Tomoko Matsui, Shuji Sugano, Shoji Usami

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The biosynthesis of the alternative oxidase protein consisting of cyanide-insensitive and salicylhydroxamic acid-sensitive respiration in Aspergillus niger was investigated. Cyanide-insensitive respiration was enhanced by addition of antimycin A to the incubation mixture, but repressed by the addition of cycloheximide, emetine, puromycin (cytosolic translation inhibitors), carbonylcyanide-m-chlorophenylhydrazone (uncoupler) and actinomycin D (transcription inhibitor). These results show that the alternative oxidase protein of A. niger was synthesized de novo in cytosol and transported in mitochondria.

Original languageEnglish
Pages (from-to)251-254
Number of pages4
JournalFEMS Microbiology Letters
Volume141
Issue number2-3
DOIs
Publication statusPublished - 1996 Aug 1

Keywords

  • Alternative oxidase
  • Antimycin A
  • Aspergillus niger
  • Cyanide-insensitive respiration
  • Salicylhydroxamic acid

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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