Enhancement and repression of cyanide-insensitive respiration in Aspergillus niger

Kotaro Kirimura, Tomoko Matsui, Shuji Sugano, Shoji Usami

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    The biosynthesis of the alternative oxidase protein consisting of cyanide-insensitive and salicylhydroxamic acid-sensitive respiration in Aspergillus niger was investigated. Cyanide-insensitive respiration was enhanced by addition of antimycin A to the incubation mixture, but repressed by the addition of cycloheximide, emetine, puromycin (cytosolic translation inhibitors), carbonylcyanide-m-chlorophenylhydrazone (uncoupler) and actinomycin D (transcription inhibitor). These results show that the alternative oxidase protein of A. niger was synthesized de novo in cytosol and transported in mitochondria.

    Original languageEnglish
    Pages (from-to)251-254
    Number of pages4
    JournalFEMS Microbiology Letters
    Volume141
    Issue number2-3
    DOIs
    Publication statusPublished - 1996 Aug 1

    Fingerprint

    Aspergillus niger
    Cyanides
    Respiration
    Emetine
    Antimycin A
    Puromycin
    Staphylococcal Protein A
    Dactinomycin
    Cycloheximide
    Cytosol
    Mitochondria
    Proteins
    alternative oxidase
    salicylhydroxamic acid

    Keywords

    • Alternative oxidase
    • Antimycin A
    • Aspergillus niger
    • Cyanide-insensitive respiration
    • Salicylhydroxamic acid

    ASJC Scopus subject areas

    • Genetics
    • Molecular Biology
    • Applied Microbiology and Biotechnology
    • Microbiology

    Cite this

    Enhancement and repression of cyanide-insensitive respiration in Aspergillus niger. / Kirimura, Kotaro; Matsui, Tomoko; Sugano, Shuji; Usami, Shoji.

    In: FEMS Microbiology Letters, Vol. 141, No. 2-3, 01.08.1996, p. 251-254.

    Research output: Contribution to journalArticle

    Kirimura, Kotaro ; Matsui, Tomoko ; Sugano, Shuji ; Usami, Shoji. / Enhancement and repression of cyanide-insensitive respiration in Aspergillus niger. In: FEMS Microbiology Letters. 1996 ; Vol. 141, No. 2-3. pp. 251-254.
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