Enhancement by prolactin of the GnRH-induced release of LH from dispersed anterior pituitary cells of the bullfrog (Rana catesbeiana)

A. Oguchi, S. Tanaka, T. Aida, K. Yamamoto, S. Kikuyama

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    The response of enzymatically dispersed anterior pituitary cells of the bullfrog (Rana catesbeiana) to gonadotropin-releasing hormone (GnRH) was studied by monitoring the release of luteinizing hormone (LH) into the culture medium. The cells responded to GnRH by releasing LH according to the incubation time and to the GnRH concentration. The responsiveness to GnRH became less conspicuous as the cell density was reduced. Addition of prolactin (PRL) to the medium enhanced the responsiveness to the secretagogue, and addition of antiserum against PRL lowered the responsiveness to a certain extent. Immunohistochemical studies of sectioned pituitaries revealed that PRL cells most frequently located in contact with LH cells. The possibility that PRL acts directly on gonadotrophs to enhance their responsiveness to GnRH was suggested.

    Original languageEnglish
    Pages (from-to)128-135
    Number of pages8
    JournalGeneral and Comparative Endocrinology
    Volume107
    Issue number1
    DOIs
    Publication statusPublished - 1997 Jul

    Fingerprint

    Prolactin-Releasing Hormone
    Rana catesbeiana
    Lithobates catesbeianus
    anterior pituitary
    gonadotropin-releasing hormone
    luteinizing hormone
    Luteinizing Hormone
    prolactin
    Gonadotropin-Releasing Hormone
    Prolactin
    cells
    Lactotrophs
    Gonadotrophs
    antiserum
    Culture Media
    Immune Sera
    culture media
    Cell Count
    monitoring

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Enhancement by prolactin of the GnRH-induced release of LH from dispersed anterior pituitary cells of the bullfrog (Rana catesbeiana). / Oguchi, A.; Tanaka, S.; Aida, T.; Yamamoto, K.; Kikuyama, S.

    In: General and Comparative Endocrinology, Vol. 107, No. 1, 07.1997, p. 128-135.

    Research output: Contribution to journalArticle

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