Enhancement by proopiomelanocortin-derived peptides of growth hormone and prolactin secretion by bullfrog pituitary cells

Tomomi Aida, Kazutoshi Yamamoto, Sakae Kikuyama

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Corticotrophs in the bullfrog (Rana catesbeiana) are situated mainly in the rostral region of the anterior lobe of the pituitary gland, which receives its blood supply primarily from the portal vessel. On the assumption that the proopiomelanocortin (POMC)-derived peptides released into the pituitary circulation may influence the function of other pituitary cells situated downstream, the effects of three POMC-derived peptides, namely, N- terminal peptide of POMC (NPP), adrenocorticotropic hormone (ACTH), and joining peptide (JP), on the secretion of growth hormone (GH) and prolactin (PRL) by bullfrog dispersed anterior pituitary cells were examined. NPP and ACTH, but not JP, stimulated the release of GH and PRL in a concentration- dependent manner. It was also found that ACTH1-17, but not α-melanocyte- stimulating hormone, was effective in enhancing GH and PRL release. A marked difference between the response to NPP and ACTH and the response to thyrotropin-releasing hormone employed as a reference secretagogue in terms of the time required for stimulating the release of GH and PRL was noted. Northern blot analysis of GH and PRL mRNA levels and radioimmunoassay for GH and PRL in the cultured cells revealed that ACTH increases the syntheses of both pituitary hormones as well. The possibility that NPP and ACTH act on neighboring cells to maintain their overall secretory function is discussed.

    Original languageEnglish
    Pages (from-to)101-109
    Number of pages9
    JournalGeneral and Comparative Endocrinology
    Volume115
    Issue number1
    DOIs
    Publication statusPublished - 1999 Jul

    Fingerprint

    pro-opiomelanocortin
    Rana catesbeiana
    peptide hormones
    Pro-Opiomelanocortin
    Peptide Hormones
    prolactin
    Prolactin
    somatotropin
    Growth Hormone
    corticotropin
    gamma-MSH
    Adrenocorticotropic Hormone
    secretion
    peptides
    Peptides
    Anterior Pituitary Gland
    cells
    melanocyte-stimulating hormone
    Corticotrophs
    thyrotropin-releasing hormone

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Enhancement by proopiomelanocortin-derived peptides of growth hormone and prolactin secretion by bullfrog pituitary cells. / Aida, Tomomi; Yamamoto, Kazutoshi; Kikuyama, Sakae.

    In: General and Comparative Endocrinology, Vol. 115, No. 1, 07.1999, p. 101-109.

    Research output: Contribution to journalArticle

    @article{6f773c53f73b48279eaffe65fe731a64,
    title = "Enhancement by proopiomelanocortin-derived peptides of growth hormone and prolactin secretion by bullfrog pituitary cells",
    abstract = "Corticotrophs in the bullfrog (Rana catesbeiana) are situated mainly in the rostral region of the anterior lobe of the pituitary gland, which receives its blood supply primarily from the portal vessel. On the assumption that the proopiomelanocortin (POMC)-derived peptides released into the pituitary circulation may influence the function of other pituitary cells situated downstream, the effects of three POMC-derived peptides, namely, N- terminal peptide of POMC (NPP), adrenocorticotropic hormone (ACTH), and joining peptide (JP), on the secretion of growth hormone (GH) and prolactin (PRL) by bullfrog dispersed anterior pituitary cells were examined. NPP and ACTH, but not JP, stimulated the release of GH and PRL in a concentration- dependent manner. It was also found that ACTH1-17, but not α-melanocyte- stimulating hormone, was effective in enhancing GH and PRL release. A marked difference between the response to NPP and ACTH and the response to thyrotropin-releasing hormone employed as a reference secretagogue in terms of the time required for stimulating the release of GH and PRL was noted. Northern blot analysis of GH and PRL mRNA levels and radioimmunoassay for GH and PRL in the cultured cells revealed that ACTH increases the syntheses of both pituitary hormones as well. The possibility that NPP and ACTH act on neighboring cells to maintain their overall secretory function is discussed.",
    author = "Tomomi Aida and Kazutoshi Yamamoto and Sakae Kikuyama",
    year = "1999",
    month = "7",
    doi = "10.1006/gcen.1999.7289",
    language = "English",
    volume = "115",
    pages = "101--109",
    journal = "General and Comparative Endocrinology",
    issn = "0016-6480",
    publisher = "Academic Press Inc.",
    number = "1",

    }

    TY - JOUR

    T1 - Enhancement by proopiomelanocortin-derived peptides of growth hormone and prolactin secretion by bullfrog pituitary cells

    AU - Aida, Tomomi

    AU - Yamamoto, Kazutoshi

    AU - Kikuyama, Sakae

    PY - 1999/7

    Y1 - 1999/7

    N2 - Corticotrophs in the bullfrog (Rana catesbeiana) are situated mainly in the rostral region of the anterior lobe of the pituitary gland, which receives its blood supply primarily from the portal vessel. On the assumption that the proopiomelanocortin (POMC)-derived peptides released into the pituitary circulation may influence the function of other pituitary cells situated downstream, the effects of three POMC-derived peptides, namely, N- terminal peptide of POMC (NPP), adrenocorticotropic hormone (ACTH), and joining peptide (JP), on the secretion of growth hormone (GH) and prolactin (PRL) by bullfrog dispersed anterior pituitary cells were examined. NPP and ACTH, but not JP, stimulated the release of GH and PRL in a concentration- dependent manner. It was also found that ACTH1-17, but not α-melanocyte- stimulating hormone, was effective in enhancing GH and PRL release. A marked difference between the response to NPP and ACTH and the response to thyrotropin-releasing hormone employed as a reference secretagogue in terms of the time required for stimulating the release of GH and PRL was noted. Northern blot analysis of GH and PRL mRNA levels and radioimmunoassay for GH and PRL in the cultured cells revealed that ACTH increases the syntheses of both pituitary hormones as well. The possibility that NPP and ACTH act on neighboring cells to maintain their overall secretory function is discussed.

    AB - Corticotrophs in the bullfrog (Rana catesbeiana) are situated mainly in the rostral region of the anterior lobe of the pituitary gland, which receives its blood supply primarily from the portal vessel. On the assumption that the proopiomelanocortin (POMC)-derived peptides released into the pituitary circulation may influence the function of other pituitary cells situated downstream, the effects of three POMC-derived peptides, namely, N- terminal peptide of POMC (NPP), adrenocorticotropic hormone (ACTH), and joining peptide (JP), on the secretion of growth hormone (GH) and prolactin (PRL) by bullfrog dispersed anterior pituitary cells were examined. NPP and ACTH, but not JP, stimulated the release of GH and PRL in a concentration- dependent manner. It was also found that ACTH1-17, but not α-melanocyte- stimulating hormone, was effective in enhancing GH and PRL release. A marked difference between the response to NPP and ACTH and the response to thyrotropin-releasing hormone employed as a reference secretagogue in terms of the time required for stimulating the release of GH and PRL was noted. Northern blot analysis of GH and PRL mRNA levels and radioimmunoassay for GH and PRL in the cultured cells revealed that ACTH increases the syntheses of both pituitary hormones as well. The possibility that NPP and ACTH act on neighboring cells to maintain their overall secretory function is discussed.

    UR - http://www.scopus.com/inward/record.url?scp=0032992423&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0032992423&partnerID=8YFLogxK

    U2 - 10.1006/gcen.1999.7289

    DO - 10.1006/gcen.1999.7289

    M3 - Article

    C2 - 10375468

    AN - SCOPUS:0032992423

    VL - 115

    SP - 101

    EP - 109

    JO - General and Comparative Endocrinology

    JF - General and Comparative Endocrinology

    SN - 0016-6480

    IS - 1

    ER -