Enhancement of biomass and lipid productivities of water surface-floating microalgae by chemical mutagenesis

Daisuke Nojima, Yuki Ishizuka, Masaki Muto, Asuka Ujiro, Fumito Kodama, Tomoko Yoshino, Yoshiaki Maeda, Tadashi Matsunaga, Tsuyoshi Tanaka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Water surface-floating microalgae have great potential for biofuel applications due to the ease of the harvesting process, which is one of the most problematic steps in conventional microalgal biofuel production. We have collected promising water surface-floating microalgae and characterized their capacity for biomass and lipid production. In this study, we performed chemical mutagenesis of two water surface-floating microalgae to elevate productivity. Floating microalgal strains AVFF007 and FFG039 (tentatively identified as Botryosphaerella sp. and Chlorococcum sp., respectively) were exposed to ethyl methane sulfonate (EMS) or 1-methyl-3-nitro-1-nitrosoguanidine (MNNG), and pale green mutants (PMs) were obtained. The most promising FFG039 PM formed robust biofilms on the surface of the culture medium, similar to those formed by wild type strains, and it exhibited 1.7-fold and 1.9-fold higher biomass and lipid productivities than those of the wild type. This study indicates that the chemical mutation strategy improves the lipid productivity of water surface-floating microalgae without inhibiting biofilm formation and floating ability.

Original languageEnglish
Article number151
JournalMarine Drugs
Volume15
Issue number6
DOIs
Publication statusPublished - 2017 Jun 1
Externally publishedYes

Fingerprint

Microalgae
Mutagenesis
Biomass
Lipids
Biofuels
Water
Biofilms
Nitrosoguanidines
Methane
Culture Media
Mutation

Keywords

  • Biofuels
  • Chemical mutagenesis
  • Lipid production
  • Microalgae
  • Water surface-floating

ASJC Scopus subject areas

  • Drug Discovery

Cite this

Nojima, D., Ishizuka, Y., Muto, M., Ujiro, A., Kodama, F., Yoshino, T., ... Tanaka, T. (2017). Enhancement of biomass and lipid productivities of water surface-floating microalgae by chemical mutagenesis. Marine Drugs, 15(6), [151]. https://doi.org/10.3390/md15060151

Enhancement of biomass and lipid productivities of water surface-floating microalgae by chemical mutagenesis. / Nojima, Daisuke; Ishizuka, Yuki; Muto, Masaki; Ujiro, Asuka; Kodama, Fumito; Yoshino, Tomoko; Maeda, Yoshiaki; Matsunaga, Tadashi; Tanaka, Tsuyoshi.

In: Marine Drugs, Vol. 15, No. 6, 151, 01.06.2017.

Research output: Contribution to journalArticle

Nojima, D, Ishizuka, Y, Muto, M, Ujiro, A, Kodama, F, Yoshino, T, Maeda, Y, Matsunaga, T & Tanaka, T 2017, 'Enhancement of biomass and lipid productivities of water surface-floating microalgae by chemical mutagenesis', Marine Drugs, vol. 15, no. 6, 151. https://doi.org/10.3390/md15060151
Nojima, Daisuke ; Ishizuka, Yuki ; Muto, Masaki ; Ujiro, Asuka ; Kodama, Fumito ; Yoshino, Tomoko ; Maeda, Yoshiaki ; Matsunaga, Tadashi ; Tanaka, Tsuyoshi. / Enhancement of biomass and lipid productivities of water surface-floating microalgae by chemical mutagenesis. In: Marine Drugs. 2017 ; Vol. 15, No. 6.
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