Enhancement of lipase production from hydrocarbons by mutation of Trichosporon fermentans

Jiacong Chen, Susumu Shimura, Kotaro Kirimura, Shoji Usami

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Lipase high-producing mutants with petroleum products as carbon sources were successfully induced from Trichosporon fermentans WU-C12 by ultraviolet (UV) light irradiation. In the first mutation step, one mutant strain, PU-30, derived from strain WU-C12 was selected. The productivity of extracellular lipase of PU-30 reached 58 units (U)/ml in the medium containing kerosene, being approximately twice the productivity of the parental strain WU-C12. In the second mutation step, the mutant strain 2PU-18 was induced from strain PU-30. In medium containing kerosene, gas oil and liquid paraffin, the 2PU-18 produced 70 U/ml, 62 U/ml and 60 U/ml of extracellular lipase, respectively. When various n-alkanes (C8-C18) were used as carbon sources, the parental strain WU-C12 produced more than 20 U/ml of lipase only from C9-C12 alkanes, but 2PU-18 could produce more than 50 U/ml of lipase from C8-C18 alkanes. When cultivated for 3 days in medium containing liquid paraffin, the activity ratios of extracellular lipase to total lipase and the values of extracellular lipase activity per dry-cell weight were 0.44 and 0.65 U/mg for WU-C12, and 0.62 and 1.82 U/mg for 2PU-18, respectively. These results indicate that the mutant strain 2PU-18 is superior in both total lipase productivity and permeability of lipase to the parental strain WU-C12 when petroleum products are used as carbon sources.

    Original languageEnglish
    Pages (from-to)714-718
    Number of pages5
    JournalApplied Microbiology and Biotechnology
    Volume38
    Issue number6
    DOIs
    Publication statusPublished - 1993 Mar

    Fingerprint

    Trichosporon
    Lipases
    Hydrocarbons
    Lipase
    Mutation
    Paraffins
    Alkanes
    Kerosene
    Mineral Oil
    Petroleum products
    Carbon
    Productivity
    Petroleum
    Liquids
    Ultraviolet Rays
    Gas oils
    Permeability
    Oils
    Gases
    Irradiation

    ASJC Scopus subject areas

    • Microbiology (medical)
    • Microbiology
    • Bioengineering
    • Biotechnology

    Cite this

    Enhancement of lipase production from hydrocarbons by mutation of Trichosporon fermentans. / Chen, Jiacong; Shimura, Susumu; Kirimura, Kotaro; Usami, Shoji.

    In: Applied Microbiology and Biotechnology, Vol. 38, No. 6, 03.1993, p. 714-718.

    Research output: Contribution to journalArticle

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    abstract = "Lipase high-producing mutants with petroleum products as carbon sources were successfully induced from Trichosporon fermentans WU-C12 by ultraviolet (UV) light irradiation. In the first mutation step, one mutant strain, PU-30, derived from strain WU-C12 was selected. The productivity of extracellular lipase of PU-30 reached 58 units (U)/ml in the medium containing kerosene, being approximately twice the productivity of the parental strain WU-C12. In the second mutation step, the mutant strain 2PU-18 was induced from strain PU-30. In medium containing kerosene, gas oil and liquid paraffin, the 2PU-18 produced 70 U/ml, 62 U/ml and 60 U/ml of extracellular lipase, respectively. When various n-alkanes (C8-C18) were used as carbon sources, the parental strain WU-C12 produced more than 20 U/ml of lipase only from C9-C12 alkanes, but 2PU-18 could produce more than 50 U/ml of lipase from C8-C18 alkanes. When cultivated for 3 days in medium containing liquid paraffin, the activity ratios of extracellular lipase to total lipase and the values of extracellular lipase activity per dry-cell weight were 0.44 and 0.65 U/mg for WU-C12, and 0.62 and 1.82 U/mg for 2PU-18, respectively. These results indicate that the mutant strain 2PU-18 is superior in both total lipase productivity and permeability of lipase to the parental strain WU-C12 when petroleum products are used as carbon sources.",
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