Enhancement of magnetic particle production by nitrate and succinate fed-batch culture of Magnetospirillum sp. AMB-1

Tadashi Matsunaga, Noriyuki Tsujimura, Shinji Kamiya

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Magnetospirillum sp. AMB-1 is a magnetic bacterium, which is capable of growing under air atmosphere. This bacterium was employed to make bacterial magnetic particles (BMPs). AMB-1 only makes BMPs during logarithmic growth phase under anaerobic conditions. Since it requires nitrate as a nitrogen source, control of nitrate concentration in the medium was necessary. The fed-batch culture of AMB-1 was carried out by adding nitric acid and succinate as nitrogen and carbon source respectively. One liter of AMB-1 culture produced 0.34 g of dry cells and 4.5 mg of BMPs. BMP production by AMB-1 cultivated in the fed-batch culture was found to be seven times higher than that cultivated in the batch culture.

Original languageEnglish
Pages (from-to)495-500
Number of pages6
JournalBiotechnology Techniques
Volume10
Issue number7
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Magnetospirillum
Batch Cell Culture Techniques
Succinic Acid
Nitrates
Bacteria
Nitrogen
Nitric Acid
Nitric acid
Carbon
Atmosphere
Air
Growth

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

Cite this

Enhancement of magnetic particle production by nitrate and succinate fed-batch culture of Magnetospirillum sp. AMB-1. / Matsunaga, Tadashi; Tsujimura, Noriyuki; Kamiya, Shinji.

In: Biotechnology Techniques, Vol. 10, No. 7, 1996, p. 495-500.

Research output: Contribution to journalArticle

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