Enhancement of nitrifying biofilm formation using selected EPS produced by heterotrophic bacteria

Satoshi Tsuneda, S. Park, H. Hayashi, J. Jung, A. Hirata

    Research output: Contribution to journalArticle

    62 Citations (Scopus)

    Abstract

    The possibility of enhancing nitrifying biofilm formation rate with the aid of selected EPS produced by heterotrophic bacteria was investigated. When EPS production characteristics were examined for four kinds of heterotrophs isolated from a domestic wastewater treatment reactor, two strains obtained from biofilms (B1, B2) exhibited a higher polysaccharide production rate than those from suspended flocs (A1, A2). Among EPS components, the concentration of uronic acids gave a good correlation with flocculation ability, which suggests that acidic polysaccharides play a major role in bioaggregate formation. Addition of 1 g/L D-glucuronic acid as an EPS substitute enhanced the homocoagulation rate of autotrophic Nitrosomonas europaea and altered its zeta potential from ñ30.4mV to +4.3mV, which indicates a possibility that particular EPS components produced by heterotrophs are utilized as neutralising reagents for nitriying biofilm formation. Moreover, when heterotrophic isolates with Nitrobacter winogradskyi were cultured in batch with fabric supports, biofilm formed on the substratum. These exprimental results suggest the application of selected EPS for enhancing nitrifying biofilm formation.

    Original languageEnglish
    Pages (from-to)197-204
    Number of pages8
    JournalWater Science and Technology
    Volume43
    Issue number6
    Publication statusPublished - 2001

    Fingerprint

    Biofilms
    biofilm
    Bacteria
    bacterium
    Polysaccharides
    polysaccharide
    Acids
    acid
    Flocculation
    Zeta potential
    flocculation
    Wastewater treatment
    rate

    Keywords

    • Biofilm formation
    • Carbohydrate
    • EPS
    • Extracellular polymeric substances
    • Heterophic bacteria
    • Nitrifying bacteria
    • Uronic acids

    ASJC Scopus subject areas

    • Water Science and Technology

    Cite this

    Enhancement of nitrifying biofilm formation using selected EPS produced by heterotrophic bacteria. / Tsuneda, Satoshi; Park, S.; Hayashi, H.; Jung, J.; Hirata, A.

    In: Water Science and Technology, Vol. 43, No. 6, 2001, p. 197-204.

    Research output: Contribution to journalArticle

    Tsuneda, Satoshi ; Park, S. ; Hayashi, H. ; Jung, J. ; Hirata, A. / Enhancement of nitrifying biofilm formation using selected EPS produced by heterotrophic bacteria. In: Water Science and Technology. 2001 ; Vol. 43, No. 6. pp. 197-204.
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