Enhancement of poly(arginyl-histidine) production by Verticillium kibiense E18

Ikumi Kurihara; Yoshitaka Ishii; Kohtaro Kirimura; Kuniki Kino

doi:10.1016/j.bej.2008.07.007

Enhancement of poly(arginyl-histidine) production by Verticillium kibiense E18

Ikumi Kurihara, Yoshitaka Ishii, Kohtaro Kirimura, Kuniki Kino

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

An ergot fungus Verticillium kibiense E18 produced two cationic peptides, ε-poly-l-lysine (ePL) and poly(l-arginyl-d-histidine) (PRH). The ePL was used as a food preservative, and it was expected that PRH would be used as a novel material, such as cationic and antimicrobial peptide. To enhance PRH production of strain E18, various culture conditions were investigated. Glucose was a suitable carbon source for PRH production, although glycerol was a suitable carbon source for growth. The cultivation temperature significantly influenced both cell growth and PRH production. The optimal temperatures for cell growth and PRH production were 28 and 30 °C, respectively. Moreover, strain E18 produced more PRH when an additional 5.0 μg/L FeSO₄·7H₂O was added to the production medium. Under optimal conditions, strain E18 enhanced PRH production, while suppressing ePL production. The maximum PRH production was 183.9 mg/L, which is approximately 60-fold higher than that of the initial culture condition.

Original language	English
Pages (from-to)	270-275
Number of pages	6
Journal	Biochemical Engineering Journal
Volume	42
Issue number	3
DOIs	https://doi.org/10.1016/j.bej.2008.07.007
Publication status	Published - 2008 Dec 1

Keywords

Antimicrobial peptide
Cationic peptide
Culture conditions
Poly(amino acid)
Poly(arginyl-histidine)
Verticillium kibiense

ASJC Scopus subject areas

Biotechnology
Environmental Engineering
Bioengineering
Biomedical Engineering

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title = "Enhancement of poly(arginyl-histidine) production by Verticillium kibiense E18",

abstract = "An ergot fungus Verticillium kibiense E18 produced two cationic peptides, ε-poly-l-lysine (ePL) and poly(l-arginyl-d-histidine) (PRH). The ePL was used as a food preservative, and it was expected that PRH would be used as a novel material, such as cationic and antimicrobial peptide. To enhance PRH production of strain E18, various culture conditions were investigated. Glucose was a suitable carbon source for PRH production, although glycerol was a suitable carbon source for growth. The cultivation temperature significantly influenced both cell growth and PRH production. The optimal temperatures for cell growth and PRH production were 28 and 30 °C, respectively. Moreover, strain E18 produced more PRH when an additional 5.0 μg/L FeSO4·7H2O was added to the production medium. Under optimal conditions, strain E18 enhanced PRH production, while suppressing ePL production. The maximum PRH production was 183.9 mg/L, which is approximately 60-fold higher than that of the initial culture condition.",

keywords = "Antimicrobial peptide, Cationic peptide, Culture conditions, Poly(amino acid), Poly(arginyl-histidine), Verticillium kibiense",

author = "Ikumi Kurihara and Yoshitaka Ishii and Kohtaro Kirimura and Kuniki Kino",

year = "2008",

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doi = "10.1016/j.bej.2008.07.007",

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AU - Kurihara, Ikumi

AU - Ishii, Yoshitaka

AU - Kirimura, Kohtaro

AU - Kino, Kuniki

PY - 2008/12/1

Y1 - 2008/12/1

N2 - An ergot fungus Verticillium kibiense E18 produced two cationic peptides, ε-poly-l-lysine (ePL) and poly(l-arginyl-d-histidine) (PRH). The ePL was used as a food preservative, and it was expected that PRH would be used as a novel material, such as cationic and antimicrobial peptide. To enhance PRH production of strain E18, various culture conditions were investigated. Glucose was a suitable carbon source for PRH production, although glycerol was a suitable carbon source for growth. The cultivation temperature significantly influenced both cell growth and PRH production. The optimal temperatures for cell growth and PRH production were 28 and 30 °C, respectively. Moreover, strain E18 produced more PRH when an additional 5.0 μg/L FeSO4·7H2O was added to the production medium. Under optimal conditions, strain E18 enhanced PRH production, while suppressing ePL production. The maximum PRH production was 183.9 mg/L, which is approximately 60-fold higher than that of the initial culture condition.

AB - An ergot fungus Verticillium kibiense E18 produced two cationic peptides, ε-poly-l-lysine (ePL) and poly(l-arginyl-d-histidine) (PRH). The ePL was used as a food preservative, and it was expected that PRH would be used as a novel material, such as cationic and antimicrobial peptide. To enhance PRH production of strain E18, various culture conditions were investigated. Glucose was a suitable carbon source for PRH production, although glycerol was a suitable carbon source for growth. The cultivation temperature significantly influenced both cell growth and PRH production. The optimal temperatures for cell growth and PRH production were 28 and 30 °C, respectively. Moreover, strain E18 produced more PRH when an additional 5.0 μg/L FeSO4·7H2O was added to the production medium. Under optimal conditions, strain E18 enhanced PRH production, while suppressing ePL production. The maximum PRH production was 183.9 mg/L, which is approximately 60-fold higher than that of the initial culture condition.

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