Abstract
An ergot fungus Verticillium kibiense E18 produced two cationic peptides, ε-poly-l-lysine (ePL) and poly(l-arginyl-d-histidine) (PRH). The ePL was used as a food preservative, and it was expected that PRH would be used as a novel material, such as cationic and antimicrobial peptide. To enhance PRH production of strain E18, various culture conditions were investigated. Glucose was a suitable carbon source for PRH production, although glycerol was a suitable carbon source for growth. The cultivation temperature significantly influenced both cell growth and PRH production. The optimal temperatures for cell growth and PRH production were 28 and 30 °C, respectively. Moreover, strain E18 produced more PRH when an additional 5.0 μg/L FeSO4·7H2O was added to the production medium. Under optimal conditions, strain E18 enhanced PRH production, while suppressing ePL production. The maximum PRH production was 183.9 mg/L, which is approximately 60-fold higher than that of the initial culture condition.
Original language | English |
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Pages (from-to) | 270-275 |
Number of pages | 6 |
Journal | Biochemical Engineering Journal |
Volume | 42 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2008 Dec 1 |
Keywords
- Antimicrobial peptide
- Cationic peptide
- Culture conditions
- Poly(amino acid)
- Poly(arginyl-histidine)
- Verticillium kibiense
ASJC Scopus subject areas
- Biotechnology
- Environmental Engineering
- Bioengineering
- Biomedical Engineering