Enhancement of recombinant antibody expression level by growth controlled medium

Le Thi Minh Phuc, Tetsuji Sasaki, Hisayo Shimizu, Nguyen Thi Minh Huyen, Nguyen Thi Thu Thuy, Le Quang Huan, Akiyoshi Taniguchi

    Research output: Contribution to journalArticle

    Abstract

    Background: Transient expression system is very widely used for protein expression including recombinant protein expression. Normally, in the commercially available expression system, cells were grown rapidly in protein expression. However, over cell growth was not only unnecessary for high level protein expression, but also induced cell death and led to protein degradation. Aims and Objectives: To overcome these limitations a new adapted culture method needs to be developed. Methods: In this work, we developed growth control medium for transient high protein expression system using NPLAd (Non-Protein and Lipid Medium Adopted) cells. With this system, cell numbers were not increased until 10 days. Results: Our results indicated that expression level in NPLAd system was 1.8 times higher than that in Free style system, which was one of the commercially available high expression systems. Conclusion: The antibody performance expressed by NPLAd system was almost the same to that of expressed by Free style system. The results suggested that NPLAd system could be useful for protein expression, such as recombinant antibody.

    Original languageEnglish
    Pages (from-to)78-85
    Number of pages8
    JournalOpen Biotechnology Journal
    Volume12
    DOIs
    Publication statusPublished - 2018 Jan 1

    Fingerprint

    Antibodies
    Proteins
    Lipids
    Growth
    Recombinant proteins
    Recombinant Proteins
    Proteolysis
    Cell growth
    Cell death
    Cell Death
    Cell Count
    Degradation

    Keywords

    • Antibody performance
    • CHO cells
    • Culture medium
    • NPLAd system
    • Protein expression
    • Recombinant antibody

    ASJC Scopus subject areas

    • Biotechnology
    • Biophysics
    • Bioengineering
    • Biochemistry
    • Biomedical Engineering

    Cite this

    Enhancement of recombinant antibody expression level by growth controlled medium. / Phuc, Le Thi Minh; Sasaki, Tetsuji; Shimizu, Hisayo; Huyen, Nguyen Thi Minh; Thuy, Nguyen Thi Thu; Huan, Le Quang; Taniguchi, Akiyoshi.

    In: Open Biotechnology Journal, Vol. 12, 01.01.2018, p. 78-85.

    Research output: Contribution to journalArticle

    Phuc, Le Thi Minh ; Sasaki, Tetsuji ; Shimizu, Hisayo ; Huyen, Nguyen Thi Minh ; Thuy, Nguyen Thi Thu ; Huan, Le Quang ; Taniguchi, Akiyoshi. / Enhancement of recombinant antibody expression level by growth controlled medium. In: Open Biotechnology Journal. 2018 ; Vol. 12. pp. 78-85.
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    AU - Sasaki, Tetsuji

    AU - Shimizu, Hisayo

    AU - Huyen, Nguyen Thi Minh

    AU - Thuy, Nguyen Thi Thu

    AU - Huan, Le Quang

    AU - Taniguchi, Akiyoshi

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