Enhancer of human polyoma JC virus contains nuclear factor I-binding sequences; analysis using mouse brain nuclear extracts

Taka aki Tamura, Takafumi Inoue, Kyosuke Nagata, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Neurotrophic human JC virus carries a 98 bp duplicate enhancer responsible for tissue-specific gene expression. DNase I footprinting studies using mouse brain nuclear extracts revealed weak (pseudo NFI motif) and strong (NFI motif) nuclear factor I-binding sequences just upstream (at 229) and in the middle (at 156 and 58) of the enhancer, respectively. In vitro transcription driven in brain extracts demonstrated that the NFI motif is a possible transcription control element. Together with previous observations(Khalili,K., Rappaport,J. and Khoury,G.(1988) EMBO J. 7, 1205-1210 (20)), the NFI motif is suggested to play an important role in early gene expression of JCV.

Original languageEnglish
Pages (from-to)419-425
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume157
Issue number2
DOIs
Publication statusPublished - 1988 Dec 15

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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