Enrichment and physiological characterization of a coldadapted nitrite-oxidizing Nitrotoga sp. from an eelgrass sediment

Kento Ishii, Hirotsugu Fujitani, Kentaro Soh, Tatsunori Nakagawa, Reiji Takahashi, Satoshi Tsuneda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Nitrite-oxidizing bacteria (NOB) are responsible for the second step of nitrification in natural and engineered ecosystems. The recently discovered genus Nitrotoga belongs to the Betaproteobacteria and potentially has high environmental importance. Although environmental clones affiliated with Nitrotoga are widely distributed, the limited number of cultivated Nitrotoga spp. results in a poor understanding of their ecophysiological features. In this study, we successfully enriched the nonmarine cold-adapted Nitrotoga sp. strain AM1 from coastal sand in an eelgrass zone and investigated its physiological characteristics. Multistep-enrichment approaches led to an increase in the abundance of AM1 to approximately 80% of the total bacterial population. AM1 was the only detectable NOB in the bacterial community. The 16S rRNA gene sequence of AM1 was 99.6% identical to that of "Candidatus Nitrotoga arctica," which was enriched from permafrost-affected soil. The highest nitrogen oxidation rate of AM1 was observed at 16°C. The half-saturation constant (Km) and the generation time were determined to be 25 μM NO2 - and 54 h, respectively. The nitrite oxidation rate of AM1 was stimulated at concentrations of <30 mM NH4Cl but completely inhibited at 50 mM NH4Cl. AM1 can grow well under specific environmental conditions, such as low temperature and in the presence of a relatively high concentration of free ammonia. These results help improve our comprehension of the functional importance of Nitrotoga.

Original languageEnglish
Article numbere00549-17
JournalApplied and Environmental Microbiology
Volume83
Issue number14
DOIs
Publication statusPublished - 2017 Jul 1

Fingerprint

Zostera marina
Nitrites
nitrites
nitrite
sediments
oxidation
Betaproteobacteria
sediment
Bacteria
Nitrification
Arctica (Arcticidae)
bacterium
beta-Proteobacteria
permafrost
generation time
bacteria
rRNA Genes
Ammonia
bacterial communities
nitrification

Keywords

  • Ammonia
  • Coastal sand
  • Cultivation
  • Enrichment
  • Microbial communities
  • Nitrification
  • Nitrite-oxidizing bacteria
  • Nitrospira
  • Nitrotoga
  • Physiology

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Enrichment and physiological characterization of a coldadapted nitrite-oxidizing Nitrotoga sp. from an eelgrass sediment. / Ishii, Kento; Fujitani, Hirotsugu; Soh, Kentaro; Nakagawa, Tatsunori; Takahashi, Reiji; Tsuneda, Satoshi.

In: Applied and Environmental Microbiology, Vol. 83, No. 14, e00549-17, 01.07.2017.

Research output: Contribution to journalArticle

Ishii, Kento ; Fujitani, Hirotsugu ; Soh, Kentaro ; Nakagawa, Tatsunori ; Takahashi, Reiji ; Tsuneda, Satoshi. / Enrichment and physiological characterization of a coldadapted nitrite-oxidizing Nitrotoga sp. from an eelgrass sediment. In: Applied and Environmental Microbiology. 2017 ; Vol. 83, No. 14.
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