Enrichment of short interspersed transposable elements to embryonic stem cell-specific hypomethylated gene regions

Hiroki Muramoto, Shintaro Yagi, Keiji Hirabayashi, Shinya Sato, Jun Ohgane, Satoshi Tanaka, Kunio Shiota

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Embryonic stem cells (ESCs) have a distinctive epigenome, which includes their genome-wide DNA methylation modification status, as represented by the ESC-specific hypomethylation of tissue-dependent and differentially methylated regions (T-DMRs) of Pou5f1 and Nanog. Here, we conducted a genome-wide investigation of sequence characteristics associated with T-DMRs that were differentially methylated between ESCs and somatic cells, by focusing on transposable elements including short interspersed elements (SINEs), long interspersed elements (LINEs) and long terminal repeats (LTRs). We found that hypomethylated T-DMRs were predominantly present in SINE-rich/LINE-poor genomic loci. The enrichment for SINEs spread over 300 kb in cis and there existed SINE-rich genomic domains spreading continuously over 1 Mb, which contained multiple hypomethylated T-DMRs. The characterization of sequence information showed that the enriched SINEs were relatively CpG rich and belonged to specific subfamilies. A subset of the enriched SINEs were hypomethylated T-DMRs in ESCs at Dppa3 gene locus, although SINEs are overall methylated in both ESCs and the liver. In conclusion, we propose that SINE enrichment is the genomic property of regions harboring hypomethylated T-DMRs in ESCs, which is a novel aspect of the ESC-specific epigenomic information.

Original languageEnglish
Pages (from-to)855-865
Number of pages11
JournalGenes to Cells
Volume15
Issue number8
DOIs
Publication statusPublished - 2010 Aug
Externally publishedYes

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DNA Transposable Elements
Embryonic Stem Cells
Genes
Genome
Terminal Repeat Sequences
DNA Methylation
Epigenomics
Liver

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Enrichment of short interspersed transposable elements to embryonic stem cell-specific hypomethylated gene regions. / Muramoto, Hiroki; Yagi, Shintaro; Hirabayashi, Keiji; Sato, Shinya; Ohgane, Jun; Tanaka, Satoshi; Shiota, Kunio.

In: Genes to Cells, Vol. 15, No. 8, 08.2010, p. 855-865.

Research output: Contribution to journalArticle

Muramoto, H, Yagi, S, Hirabayashi, K, Sato, S, Ohgane, J, Tanaka, S & Shiota, K 2010, 'Enrichment of short interspersed transposable elements to embryonic stem cell-specific hypomethylated gene regions', Genes to Cells, vol. 15, no. 8, pp. 855-865. https://doi.org/10.1111/j.1365-2443.2010.01423.x
Muramoto, Hiroki ; Yagi, Shintaro ; Hirabayashi, Keiji ; Sato, Shinya ; Ohgane, Jun ; Tanaka, Satoshi ; Shiota, Kunio. / Enrichment of short interspersed transposable elements to embryonic stem cell-specific hypomethylated gene regions. In: Genes to Cells. 2010 ; Vol. 15, No. 8. pp. 855-865.
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