Enzymatic biofuel cell with self-regulating enzyme-nanotube ensemble films

Takeo Miyake, Syuhei Yoshino, Takeo Yamada, Kenji Hata, Matsuhiko Nishizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Nanostructured carbons have been widely used for fabricating enzyme-modified electrodes due to their large specific surface area. However, because they are random aggregates of particular or tubular nanocarbons, the post-modification of enzymes to their intra-nanospace is generally hard to control. Here, we describe a free-standing film of carbon nanotube forest (CNTF) that can form a hybrid ensemble with enzymes through liquid-induced shrinkage. This provides in-situ regulation of its intra-nanospace (inter CNT pitch) to the size of enzymes, and eventually serves as a highly active electrode. The CNTF ensemble with fructose dehydrogenase (FDH) showed the oxidation current density of 16 mA cm -2 in stirred 200 mM fructose solution. The power density of a biofuel cell using the FDH-CNTF anode and the Laccase-CNTF cathode reached 1.8 mW cm -2 (at 0.45 V) in the stirred oxygenic fructose solution, more than 80 % of which could be maintained after continuous operation for 24 h. Application of the free-standing, flexible character of the enzyme-CNTF ensemble electrodes is demonstrated via their use in the patch or wound form.

Original languageEnglish
Title of host publicationMEMS, BioMEMS and Bioelectronics - Materials and Devices
Number of pages6
Publication statusPublished - 2012
Externally publishedYes
Event2011 MRS Fall Meeting - Boston, MA
Duration: 2011 Nov 282011 Dec 3


Other2011 MRS Fall Meeting
CityBoston, MA


ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Miyake, T., Yoshino, S., Yamada, T., Hata, K., & Nishizawa, M. (2012). Enzymatic biofuel cell with self-regulating enzyme-nanotube ensemble films. In MEMS, BioMEMS and Bioelectronics - Materials and Devices (Vol. 1415, pp. 87-92) https://doi.org/10.1557/opl.2012.208