Enzymatic elimination of hydrogen peroxide by a methemoglobin/L-tyrosine system

Tomoyasu Atoji, Hirotaka Yatami, Motonari Aihara, Shinji Takeoka

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    We studied the peroxidase activity of ferrylhemoglobin radical (Hb(Fe4+ = O*)) generated by the reaction of metHb (Hb(Fe3+)) with hydrogen peroxide (H2O2). To clarify the behaviors of ferrylHb radical, it was isolated from the reaction mixture of metHb and H2O2 by GPC at 4°C. The radical species underwent rapid autoreduction to metHb at 37°C accompanied with denaturation; however, it was stable for several minutes at 4°C. In ESR measurements, the signal of the ferrylHb radical immediately disappeared in the presence of l-Tyrosine (l-Tyr), and simultaneously, the signal of the ferric heme increased. This suggested that the ferrylHb radical immediately converted to metHb by l-Tyr even at 4°C. Furthermore, dimerized l-Tyr was detected in the reaction mixture. This showed that the ferrylHb radical was reduced to metHb by electron donation from l-Tyr. The enzymatic reaction using l-Tyr as the substrate resulted in the elimination of H2O2 in this system.

    Original languageEnglish
    Pages (from-to)555-567
    Number of pages13
    JournalArtificial Cells, Blood Substitutes, and Biotechnology
    Volume35
    Issue number6
    DOIs
    Publication statusPublished - 2007 Nov

    Fingerprint

    Methemoglobin
    Hydrogen peroxide
    Hydrogen Peroxide
    Tyrosine
    Denaturation
    Paramagnetic resonance
    Electrons
    Substrates
    Heme
    Peroxidase
    ferrylhemoglobin

    Keywords

    • Ferrylhemoglobin radical
    • Hemoglobin vesicle
    • Hydrogen peroxide
    • L-Tyrosine
    • Methemoglobin
    • Peroxidase activity

    ASJC Scopus subject areas

    • Biotechnology
    • Biomedical Engineering
    • Hematology

    Cite this

    Enzymatic elimination of hydrogen peroxide by a methemoglobin/L-tyrosine system. / Atoji, Tomoyasu; Yatami, Hirotaka; Aihara, Motonari; Takeoka, Shinji.

    In: Artificial Cells, Blood Substitutes, and Biotechnology, Vol. 35, No. 6, 11.2007, p. 555-567.

    Research output: Contribution to journalArticle

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