Enzymatic Kolbe-Schmitt Reaction for the Syntheses of Value-Added Compounds: Use of Biocatalysts for Carboxylation of Organic Compounds and Bioproduction. Use of Biocatalysts for Carboxylation of Organic Compounds and Bioproduction.

Kotaro Kirimura, Yoshitaka Ishii

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)


Biocatalytic reactions by enzymes have become significant for bioproduction of value-added compounds since they enable selective production of the desired compounds due to reaction-, substrate- and regio-specific enzymatic activities. In this review, the enzymatic carboxylation reactions applicable to bioproduction of aryl hydroxycarboxylic acids are discussed. Especially, the reversible decarboxylases found in microorganisms are important for bioproduction. These enzymes catalyze reverse reaction, regio-specific carboxylation, without coenzymes or ATP, and are applicable to direct carboxylation of specific compounds in solutions containing KHCO3 or NaHCO3. Salicylate decarboxylase (EC is one of the key reversible decarboxylases, catalyzing the synthesis of salicylic acid from phenol, by the enzymatic Kolbe-Schmitt reaction. The possible applications of the enzymatic Kolbe-Schmitt reaction and the future direction of this research field are discussed.

Original languageEnglish
Title of host publicationFuture Directions in Biocatalysis
Subtitle of host publicationSecond Edition
Number of pages13
ISBN (Print)9780444637437
Publication statusPublished - 2017 Aug 15



  • Biocatalytic CO fixation
  • Bioproduction
  • Carboxylation by biocatalysts
  • Enzymatic Kolbe-Schmitt reaction
  • Reversible

ASJC Scopus subject areas

  • Engineering(all)
  • Chemical Engineering(all)

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