Enzymatic replacement of nucleotide sequences in the D-loop of T. utilis tRNATyr and its effect on aminoacylation.

Takashi Ohyama, K. Nishikawa, S. Takemura

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Abstract

An efficient method for replacement of nucleotide sequences in the D-loop of T. utilis tRNATyr has been developed. An abnormal tRNATyr lacking in tetranucleotide D16-D-Gm-G19 in its D-loop has been reconstructed by this method and shown to accept tyrosine to about 55% of the aminoacylation level observed for intact tRNATyr. This suggests that the deleted sequence itself is not essential for recognition by TyrRS but a conformational instability of the tRNA possibly caused by the disruption of tertiary interactions between the D-loop and T psi C-loop might have influenced the forward reaction rate leading to the decreased level of aminoacylation.

Original languageEnglish
Pages (from-to)137-140
Number of pages4
JournalNucleic acids symposium series
Issue number12
Publication statusPublished - 1983
Externally publishedYes

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RNA, Transfer, Tyr
Aminoacylation
Transfer RNA
Tyrosine

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Enzymatic replacement of nucleotide sequences in the D-loop of T. utilis tRNATyr and its effect on aminoacylation. / Ohyama, Takashi; Nishikawa, K.; Takemura, S.

In: Nucleic acids symposium series, No. 12, 1983, p. 137-140.

Research output: Contribution to journalArticle

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