Enzymatic synthesis of terpenyl esters by transesterification with fatty acid vinyl esters as acyl donors by Trichosporon fermentans lipase

H. Nakagawa, S. Watanabe, S. Shimura, Kotaro Kirimura, S. Usami

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Enzymatic synthesis of terpenyl esters by esterification or transesterification with fatty acid vinyl esters as acyl donors by celite-adsorbed lipase of Trichosporon fermentans was investigated. In direct esterification of geraniol, the lipase showed high reactivity toward fatty acids with carbon chains longer than C-8, but little reactivity toward fatty acids with shorter chains. With fatty acid vinyl esters as acyl donors, the lipase catalysed the synthesis of geranyl and citronellyl esters with carbon chains shorter than C-6 in with yields of >90% molar conversion. Time course, effects of added water, temperature and substrate concentration were studied for the synthesis of geranyl acetate. Molar conversion yield reached 97.5% after 5 h incubation at 30-40 °C with the addition of 3% water. In this reaction, no inhibition by substrates such as geraniol and vinyl acetate was observed.

    Original languageEnglish
    Pages (from-to)219-222
    Number of pages4
    JournalWorld Journal of Microbiology and Biotechnology
    Volume14
    Issue number2
    DOIs
    Publication statusPublished - 1998

    Fingerprint

    Trichosporon
    transesterification
    Transesterification
    Lipase
    Esters
    Fatty Acids
    esters
    fatty acids
    synthesis
    geraniol
    Esterification
    esterification
    Carbon
    acetates
    Diatomaceous Earth
    Water
    Volatile Fatty Acids
    carbon
    Substrates
    water temperature

    Keywords

    • Citronellyl ester
    • Fatty acid vinyl ester
    • Geranyl ester
    • Lipase
    • Transesterification
    • Trichosporon fermentans

    ASJC Scopus subject areas

    • Food Science
    • Plant Science
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Microbiology

    Cite this

    Enzymatic synthesis of terpenyl esters by transesterification with fatty acid vinyl esters as acyl donors by Trichosporon fermentans lipase. / Nakagawa, H.; Watanabe, S.; Shimura, S.; Kirimura, Kotaro; Usami, S.

    In: World Journal of Microbiology and Biotechnology, Vol. 14, No. 2, 1998, p. 219-222.

    Research output: Contribution to journalArticle

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    AU - Shimura, S.

    AU - Kirimura, Kotaro

    AU - Usami, S.

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    KW - Transesterification

    KW - Trichosporon fermentans

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