Enzyme-treated asparagus extract attenuates hydrogen peroxide-induced matrix metalloproteinase-9 expression in murine skin fibroblast L929 cells

Ken Shirato, Jun Takanari, Junetsu Ogasawara, Takuya Sakurai, Kazuhiko Imaizumi, Hideki Ohno, Takako Kizaki

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Enzyme-treated asparagus extract (ETAS) exerts a wide variety of beneficial biological actions including facilitating anti-cortisol stress and neurological antiaging responses. However, the anti-skin aging effects of ETAS remain to be elucidated. Reactive oxygen species (ROS) play pivotal roles in skin aging. Increased ROS levels in fibroblasts in response to ultraviolet irradiation activate c-Jun N-terminal kinase (JNK) and its downstream transcription factor activator protein-1 (AP-1), and the resultant gene expression of matrix metalloproteinase (MMP) isoforms accelerates collagen breakdown in the dermis. Therefore, we explored whether ETAS has anti-skin aging effects by attenuating the oxidative stress responses in fibroblasts. Simultaneous treatment of murine skin L929 fibroblasts with hydrogen peroxide (H2O2) and either ETAS or dextrin showed that ETAS significantly suppressed H2O2-induced expression of MMP-9 mRNA as measured by real-time polymerase chain reaction. ETAS also clearly suppressed H2O2-stimulated phosphorylation of c-Jun (AP-1 subunit) and JNK as determined by Western blot. However, ETAS did not affect the increased amounts of carbonyl proteins in response to H2O2, also as determined by Western blotting. These results suggest that ETAS diminishes cellular responsiveness to ROS but does not scavenge ROS. Thus, ETAS has the potential to prevent skin aging through attenuating the oxidative stress responses in dermal fibroblasts.

    Original languageEnglish
    Pages (from-to)677-680
    Number of pages4
    JournalNatural Product Communications
    Volume11
    Issue number5
    Publication statusPublished - 2016

    Fingerprint

    gelatinase B
    Matrix Metalloproteinase 9
    skin (animal)
    Hydrogen Peroxide
    fibroblasts
    hydrogen peroxide
    Fibroblasts
    Skin
    Skin Aging
    mice
    extracts
    Enzymes
    enzymes
    reactive oxygen species
    Reactive Oxygen Species
    JNK Mitogen-Activated Protein Kinases
    Transcription Factor AP-1
    mitogen-activated protein kinase
    stress response
    Western blotting

    Keywords

    • C-Jun N-terminal kinase
    • Enzyme-treated asparagus extract
    • Fibroblast
    • Hydrogen peroxide
    • Matrix metalloproteinase-9
    • Oxidative stress response

    ASJC Scopus subject areas

    • Medicine(all)
    • Pharmacology
    • Plant Science
    • Drug Discovery
    • Complementary and alternative medicine

    Cite this

    Shirato, K., Takanari, J., Ogasawara, J., Sakurai, T., Imaizumi, K., Ohno, H., & Kizaki, T. (2016). Enzyme-treated asparagus extract attenuates hydrogen peroxide-induced matrix metalloproteinase-9 expression in murine skin fibroblast L929 cells. Natural Product Communications, 11(5), 677-680.

    Enzyme-treated asparagus extract attenuates hydrogen peroxide-induced matrix metalloproteinase-9 expression in murine skin fibroblast L929 cells. / Shirato, Ken; Takanari, Jun; Ogasawara, Junetsu; Sakurai, Takuya; Imaizumi, Kazuhiko; Ohno, Hideki; Kizaki, Takako.

    In: Natural Product Communications, Vol. 11, No. 5, 2016, p. 677-680.

    Research output: Contribution to journalArticle

    Shirato, K, Takanari, J, Ogasawara, J, Sakurai, T, Imaizumi, K, Ohno, H & Kizaki, T 2016, 'Enzyme-treated asparagus extract attenuates hydrogen peroxide-induced matrix metalloproteinase-9 expression in murine skin fibroblast L929 cells', Natural Product Communications, vol. 11, no. 5, pp. 677-680.
    Shirato, Ken ; Takanari, Jun ; Ogasawara, Junetsu ; Sakurai, Takuya ; Imaizumi, Kazuhiko ; Ohno, Hideki ; Kizaki, Takako. / Enzyme-treated asparagus extract attenuates hydrogen peroxide-induced matrix metalloproteinase-9 expression in murine skin fibroblast L929 cells. In: Natural Product Communications. 2016 ; Vol. 11, No. 5. pp. 677-680.
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