Epigenetic marks by DNA methylation specific to stem, germ and somatic cells in mice

Kunio Shiota, Yasushi Kogo, Jun Ohgane, Takuya Imamura, Atsushi Urano, Koichiro Nishino, Satoshi Tanaka, Naka Hattori

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

Background: DNA methylation is involved in many gene functions such as gene-silencing, X-inactivation, imprinting and stability of the gene. We recently found that some CpG islands had a tissue-dependent and differentially methylated region (T-DMR) in normal tissues, raising the possibility that there may be more CpG islands capable of differential methylation. Results: We investigated the genome-wide DNA methylation pattern of CpG islands by restriction landmark genomic scanning (RLGS) in mouse stem cells (ES, EG and trophoblast stem) before and after differentiation, and sperm as well as somatic tissues. A total of 247 spots out of 1500 (16%) showed differences in the appearance of their RLGS profiles, indicating that CpG islands having T-DMR were numerous and widespread. The methylation pattern was specific, and varied in a precise manner according to cell lineage, tissue type and during cell differentiation. Conclusions: Genomic loci with altered methylation status seem to be more common than has hitherto been realized. The formation of DNA methylation patterns at CpG islands is one of the epigenetic events which underlies the production of various cell types in the body. These findings should have implications for the use of embryonic stem cells and cells derived from them therapeutically, and also for the cloning of animals by the transfer of somatic cell nuclei.

Original languageEnglish
Pages (from-to)961-969
Number of pages9
JournalGenes to Cells
Volume7
Issue number9
DOIs
Publication statusPublished - 2002 Sep
Externally publishedYes

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CpG Islands
DNA Methylation
Epigenomics
Germ Cells
Methylation
X Chromosome Inactivation
Trophoblasts
Gene Silencing
Cell Lineage
Embryonic Stem Cells
Cell Nucleus
Genes
Spermatozoa
Organism Cloning
Cell Differentiation
Stem Cells
Genome

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Shiota, K., Kogo, Y., Ohgane, J., Imamura, T., Urano, A., Nishino, K., ... Hattori, N. (2002). Epigenetic marks by DNA methylation specific to stem, germ and somatic cells in mice. Genes to Cells, 7(9), 961-969. https://doi.org/10.1046/j.1365-2443.2002.00574.x

Epigenetic marks by DNA methylation specific to stem, germ and somatic cells in mice. / Shiota, Kunio; Kogo, Yasushi; Ohgane, Jun; Imamura, Takuya; Urano, Atsushi; Nishino, Koichiro; Tanaka, Satoshi; Hattori, Naka.

In: Genes to Cells, Vol. 7, No. 9, 09.2002, p. 961-969.

Research output: Contribution to journalArticle

Shiota, K, Kogo, Y, Ohgane, J, Imamura, T, Urano, A, Nishino, K, Tanaka, S & Hattori, N 2002, 'Epigenetic marks by DNA methylation specific to stem, germ and somatic cells in mice', Genes to Cells, vol. 7, no. 9, pp. 961-969. https://doi.org/10.1046/j.1365-2443.2002.00574.x
Shiota, Kunio ; Kogo, Yasushi ; Ohgane, Jun ; Imamura, Takuya ; Urano, Atsushi ; Nishino, Koichiro ; Tanaka, Satoshi ; Hattori, Naka. / Epigenetic marks by DNA methylation specific to stem, germ and somatic cells in mice. In: Genes to Cells. 2002 ; Vol. 7, No. 9. pp. 961-969.
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