Epigenetics: The DNA Methylation Profile of Tissue-Dependent and Differentially Methylated Regions in Cells

J. Ohgane, S. Yagi, K. Shiota

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Methylation of DNA, which occurs at cytosines of CpG sequences, is a unique chemical modification of the vertebrate genome. Methylation patterns can be copied to daughter DNA after mitosis; thus DNA methylation has been suggested to act as a "cellular memory of the genome function". Genome-wide analysis of DNA methylation revealed that there are numerous tissue-dependent differentially methylated regions (T-DMRs) in unique sequences of the mammalian genome. There are T-DMRs in both CpG-rich and -poor sequences. Methylation of T-DMRs is responsible for gene-silencing and chromatin structure change. Each tissue/cell type has a unique DNA methylation profile that consists of methylation patterns of numerous loci in the genome. DNA methylation profiles are not associated with bulk DNA, which is mainly comprised of repetitive sequences. Disruption of DNA methylation profiles putatively produce abnormal cells and tissues. Cloned mice produced by somatic nuclear transfer are associated with aberrant DNA methylation profiles. Tissue/cell type-specific DNA methylation profiles can provide a novel viewpoint for understanding normal and aberrant development, in terms of both differentiation and reproduction.

Original languageEnglish
Pages (from-to)29-35
Number of pages7
JournalPlacenta
Volume29
Issue numberSUPPL.
DOIs
Publication statusPublished - 2008 Mar
Externally publishedYes

Fingerprint

DNA Methylation
Epigenomics
Genome
Methylation
Nucleic Acid Repetitive Sequences
Cytosine
DNA
Gene Silencing
Mitosis
Chromatin
Reproduction
Vertebrates

Keywords

  • DNA methylation
  • DNA methylation profile
  • Epigenetics
  • T-DMRs
  • Tissue-dependent and differentially methylated regions

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Developmental Biology

Cite this

Epigenetics : The DNA Methylation Profile of Tissue-Dependent and Differentially Methylated Regions in Cells. / Ohgane, J.; Yagi, S.; Shiota, K.

In: Placenta, Vol. 29, No. SUPPL., 03.2008, p. 29-35.

Research output: Contribution to journalArticle

Ohgane, J. ; Yagi, S. ; Shiota, K. / Epigenetics : The DNA Methylation Profile of Tissue-Dependent and Differentially Methylated Regions in Cells. In: Placenta. 2008 ; Vol. 29, No. SUPPL. pp. 29-35.
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