Abstract
The mRNA expression of the sialyltransferase genes is regulated in a cell type specific manner. We show here the epithelium cell-specific transcriptional regulation of the human Galβ1, 3GalNAc/Galβ1, 4 GlcNAc α2,3-sialyltransferase gene (hST3Gal IV). Using a luciferase assay, we identified a functional DNA portion within hST3Gal IV genomic DNA that confers an epithelial cell line specific enhancer, located in nucleotide number (nt) -520 to -420 within the B3 promoter. This element contains two sequences similar to AP2 recognition motifs. Co-transfection with an AP2 expression vector stimulated the enhancer activity of nt -520 to -420 element eight-fold compared with that using parental vector. Site-directed mutagenesis of AP2 sites showed that two AP2 motifs are essential for enhancer activity in HeLa cells. These results suggest that AP2 plays a critical role in the epithelium-cell specific transcriptional regulation of the hST3Gal IV gene.
| Original language | English |
|---|---|
| Pages (from-to) | 516-522 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 257 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1999 Apr 13 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Epithelial-cell-specific transcriptional regulation of human Galβ1, 3GalNAc/Galβ1, 4GlcNAc α2,3-sialyltransferase (hST3Gal IV) gene. / Taniguchi, Akiyoshi; Matsumoto, Kojiro.
In: Biochemical and Biophysical Research Communications, Vol. 257, No. 2, 13.04.1999, p. 516-522.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Epithelial-cell-specific transcriptional regulation of human Galβ1, 3GalNAc/Galβ1, 4GlcNAc α2,3-sialyltransferase (hST3Gal IV) gene
AU - Taniguchi, Akiyoshi
AU - Matsumoto, Kojiro
PY - 1999/4/13
Y1 - 1999/4/13
N2 - The mRNA expression of the sialyltransferase genes is regulated in a cell type specific manner. We show here the epithelium cell-specific transcriptional regulation of the human Galβ1, 3GalNAc/Galβ1, 4 GlcNAc α2,3-sialyltransferase gene (hST3Gal IV). Using a luciferase assay, we identified a functional DNA portion within hST3Gal IV genomic DNA that confers an epithelial cell line specific enhancer, located in nucleotide number (nt) -520 to -420 within the B3 promoter. This element contains two sequences similar to AP2 recognition motifs. Co-transfection with an AP2 expression vector stimulated the enhancer activity of nt -520 to -420 element eight-fold compared with that using parental vector. Site-directed mutagenesis of AP2 sites showed that two AP2 motifs are essential for enhancer activity in HeLa cells. These results suggest that AP2 plays a critical role in the epithelium-cell specific transcriptional regulation of the hST3Gal IV gene.
AB - The mRNA expression of the sialyltransferase genes is regulated in a cell type specific manner. We show here the epithelium cell-specific transcriptional regulation of the human Galβ1, 3GalNAc/Galβ1, 4 GlcNAc α2,3-sialyltransferase gene (hST3Gal IV). Using a luciferase assay, we identified a functional DNA portion within hST3Gal IV genomic DNA that confers an epithelial cell line specific enhancer, located in nucleotide number (nt) -520 to -420 within the B3 promoter. This element contains two sequences similar to AP2 recognition motifs. Co-transfection with an AP2 expression vector stimulated the enhancer activity of nt -520 to -420 element eight-fold compared with that using parental vector. Site-directed mutagenesis of AP2 sites showed that two AP2 motifs are essential for enhancer activity in HeLa cells. These results suggest that AP2 plays a critical role in the epithelium-cell specific transcriptional regulation of the hST3Gal IV gene.
UR - http://www.scopus.com/inward/record.url?scp=0033551130&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033551130&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1999.0509
DO - 10.1006/bbrc.1999.0509
M3 - Article
C2 - 10198243
AN - SCOPUS:0033551130
VL - 257
SP - 516
EP - 522
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -