Equilibrium and transition between single- and double-headed binding of kinesin as revealed by single-molecule mechanics

Kenji Kawaguchi, Sotaro Uemura, Shin'Ichi Ishiwata

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    39 Citations (Scopus)

    Abstract

    Kinesin is a processive motor protein that "walks" on a microtubule toward its plus end. We reported previously that the distribution of unbinding force and elastic modulus for a single kinesin-microtubule complex was either unimodal or bimodal depending on the nucleotide states of the kinesin heads, hence showing that the kinesin may bind the microtubule either with one head or with both heads at once. Here, we found that the shape of the unbinding-force distribution depends both on the loading rate and on the manner of loading not only in the presence of AMP-PNP but also in the absence of nucleotides. Irrespective of the nucleotide state and the loading conditions examined here, the unbinding force obtained by loading directed toward the minus end of microtubule was 45% greater than that for plus end-directed loading. These results could be explained by a model in which equilibrium exists between single- and double-headed binding and the load (F) dependence of lifetime, τ(F), of each binding is expressed by τ(F) = τ(0)exp(-Fd/kBT), where τ(0) is the lifetime without external load and d a characteristic distance, both of which depend on single- or double-headed binding, kB, the Boltzmann constant and T, the absolute temperature. The model analysis showed that the forward and backward rates of transition from single- to doubleheaded binding are 2 and 0.2/s for the AMP-PNP state, and 70 and 7/s for the nucleotide-free state. Moreover, in the presence of AMP-PNP, we detected the moment of transition from single- to double-headed binding through an abrupt increase in the elastic modulus and estimated the transition rate to be ∼1/s, which is consistent with the model analysis.

    Original languageEnglish
    Pages (from-to)1103-1113
    Number of pages11
    JournalBiophysical Journal
    Volume84
    Issue number2 I
    Publication statusPublished - 2003 Feb 1

    ASJC Scopus subject areas

    • Biophysics

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