TY - JOUR
T1 - Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts
AU - Wakayama, Sayaka
AU - Jakt, Martin L.
AU - Suzuki, Masako
AU - Araki, Ryoko
AU - Hikichi, Takafusa
AU - Kishigami, Satoshi
AU - Ohta, Hiroshi
AU - Van Thuan, Nguyen
AU - Mizutani, Eiji
AU - Sakaide, Yuko
AU - Senda, Sho
AU - Tanaka, Satoshi
AU - Okada, Mitsuhiro
AU - Miyake, Masashi
AU - Abe, Masumi
AU - Nishikawa, Shin Ichi
AU - Shiota, Kunio
AU - Wakayama, Teruhiko
PY - 2006
Y1 - 2006
N2 - Therapeutic cloning, whereby nuclear transfer (NT) is used to generate embryonic stem cells (ESCs) from blastocysts, has been demonstrated successfully in mice and cattle. However, if NT-ESCs have abnormalities, such as those associated with the offspring produced by reproductive cloning, their scientific and medical utilities might prove limited. To evaluate the characteristics of NT-ESCs, we established more than 150 NT-ESC lines from adult somatic cells of several mouse strains. Here, we show that these NT-ESCs were able to differentiate into all functional embryonic tissues in vivo. Moreover, they were identical to blastocyst-derived ESCs in terms of their expression of pluripotency markers in the presence of tissue-dependent differentially DNA methylated regions, in DNA microarray profiles, and in high-coverage gene expression profiling. Importantly, the NT procedure did not cause irreversible damage to the nuclei. These similarities of NT-ESCs and ESCs indicate that murine therapeutic cloning by somatic cell NT can provide a reliable model for preclinical stem cell research.
AB - Therapeutic cloning, whereby nuclear transfer (NT) is used to generate embryonic stem cells (ESCs) from blastocysts, has been demonstrated successfully in mice and cattle. However, if NT-ESCs have abnormalities, such as those associated with the offspring produced by reproductive cloning, their scientific and medical utilities might prove limited. To evaluate the characteristics of NT-ESCs, we established more than 150 NT-ESC lines from adult somatic cells of several mouse strains. Here, we show that these NT-ESCs were able to differentiate into all functional embryonic tissues in vivo. Moreover, they were identical to blastocyst-derived ESCs in terms of their expression of pluripotency markers in the presence of tissue-dependent differentially DNA methylated regions, in DNA microarray profiles, and in high-coverage gene expression profiling. Importantly, the NT procedure did not cause irreversible damage to the nuclei. These similarities of NT-ESCs and ESCs indicate that murine therapeutic cloning by somatic cell NT can provide a reliable model for preclinical stem cell research.
KW - Cloning
KW - Embryonic stem
KW - Nuclear transfer
KW - Reprogramming
UR - http://www.scopus.com/inward/record.url?scp=33748330467&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748330467&partnerID=8YFLogxK
U2 - 10.1634/stemcells.2005-0537
DO - 10.1634/stemcells.2005-0537
M3 - Article
C2 - 16690779
AN - SCOPUS:33748330467
VL - 24
SP - 2023
EP - 2033
JO - Stem Cells
JF - Stem Cells
SN - 1066-5099
IS - 9
ER -