Escherichia coli tRNAs are resistant to the hyperprocessing reaction of homologous E. coli ribonuclease P ribozyme

Terumichi Tanaka, Tomoaki Ando, Etsuko Sakai, Taka Aki Hahisba, Yoshiaki Hori, Yo Kikuchi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bacterial ribonuclease P RNA ribozyme can do the hyperprocessing reaction, the internal cleavage reaction of some floppy eukaryotic tRNAs. The hyperprocessing reaction can be used as a detection tool to examine the stability of the cloverleaf shape of tRNA. Until now, the hyperprocessing reaction has been observed in the heterologous combination of eukaryotic tRNAs and bacterial RNase P enzymes. In this paper, we examined the hyperprocessing reaction of Escherichia coli tRNAs by homologous E. coli RNase P, to find that these homologous tRNAs were resistant to the toxic hyperprocessing reaction. Our results display the evidence for molecular co-evolution between homologous tRNAs and RNase P in the bacterium E. coli.

Original languageEnglish
Pages (from-to)1172-1176
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number5
Publication statusPublished - 2003 May
Externally publishedYes

Keywords

  • Escherichia coli
  • Evolution
  • Hyperprocessing
  • Ribonuclease P
  • tRNA

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Chemistry (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

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