We present ESPnet-SE, which is designed for the quick development of speech enhancement and speech separation systems in a single framework, along with the optional downstream speech recognition module. ESPnet-SE is a new project which integrates rich automatic speech recognition related models, resources and systems to support and validate the proposed front-end implementation (i.e. speech enhancement and separation). It is capable of processing both single-channel and multi-channel data, with various functionalities including dereverberation, denoising and source separation. We provide all-in-one recipes including data pre-processing, feature extraction, training and evaluation pipelines for a wide range of benchmark datasets. This paper describes the design of the toolkit, several important functionalities, especially the speech recognition integration, which differentiates ESPnet-SE from other open source toolkits, and experimental results with major benchmark datasets.