Establishment of a new detection system for the dimerization of IRE1α by BiFC assay

Satoko Shinjo, Etsu Tashiro, Masaya Imoto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We developed a new detection system for the activation of an endoplasmic reticulum (ER) stress sensor, inositol requiring kinase 1 α (IRE1α), by evaluating dimerization of it by bimolecular fluorescence complementation (BiFC) assay. By detecting the fluorescence derived from the reconstituted cerulean, this assay system enabled us to distinguish the activation behaviors of IRE1α as to ER stress-inducing compounds.

Original languageEnglish
Pages (from-to)1333-1336
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number6
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Endoplasmic Reticulum Stress
Dimerization
Inositol
Assays
Phosphotransferases
Fluorescence
Chemical activation
Sensors

Keywords

  • (IRE1α)
  • Bimolecular fluorescent complementation (BiFC) assay
  • Imaging
  • Inositol requiring kinase 1 α
  • Small molecule
  • Unfolded protein response (UPR)

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Establishment of a new detection system for the dimerization of IRE1α by BiFC assay. / Shinjo, Satoko; Tashiro, Etsu; Imoto, Masaya.

In: Bioscience, Biotechnology and Biochemistry, Vol. 77, No. 6, 2013, p. 1333-1336.

Research output: Contribution to journalArticle

Shinjo, Satoko ; Tashiro, Etsu ; Imoto, Masaya. / Establishment of a new detection system for the dimerization of IRE1α by BiFC assay. In: Bioscience, Biotechnology and Biochemistry. 2013 ; Vol. 77, No. 6. pp. 1333-1336.
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