Establishment of a total liquid ventilation system using saline-based oxygen micro/nano-bubble dispersions in rats

Kenta Kakiuchi, Kenichi Matsuda, Norikazu Harii, Keitaro Sou, Junko Aoki, Shinji Takeoka

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    3 Citations (Scopus)


    Micro/nano-bubbles are practical nanomaterials designed to increase the gas content in liquids. We attempted to use oxygen micro/nano-bubble dispersions as an oxygen-rich liquid as a means for total liquid ventilation. To determine the oxygen content in the bubble dispersion, a new method based on a spectrophotometric change between oxy- and deoxy-hemoglobin was established. The oxygen micro/nano-bubble dispersion was supplied to an experimental total ventilation liquid in anesthetic rats. Though the amount of dissolving oxygen was as low as 6 mg/L in physiological saline, the oxygen content in the oxygen micro/nano-bubble dispersion was increased to 45 mg/L. The positive correlation between the oxygen content and the life-saving time under liquid ventilation clearly indicates that the life-saving time is prolonged by increasing the oxygen content in the oxygen micro/nano-bubble dispersion. This is the first report indicating that the oxygen micro/nano-bubbles containing a sufficient amount of oxygen are useful in producing oxygen-rich liquid for the process of liquid ventilation.

    Original languageEnglish
    Pages (from-to)220-227
    Number of pages8
    JournalJournal of Artificial Organs
    Issue number3
    Publication statusPublished - 2015 Apr 9



    • Liquid ventilation
    • Lung
    • Micro/nano-bubbles
    • Oxygen

    ASJC Scopus subject areas

    • Biomaterials
    • Biomedical Engineering
    • Cardiology and Cardiovascular Medicine
    • Medicine (miscellaneous)

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