Abstract
Ameloblastomas are slowly growing, locally invasive neoplasms with a potentially destructive behaviour. The molecular mechanisms that regulate the cell growth and invasion of ameloblastoma cells are unknown. Because ameloblastoma cells placed in culture have a very limited lifespan, the establishment of immortalized clones of ameloblastoma cells would aid its study. We produced an immortalized ameloblastoma cell line (AM-1) using human papillomavirus type-16. This cell line maintains epithelial cell morphology and expresses cytokeratins K8, K14, K18, K19. Furthermore, bcl-2 protein, which prevents apoptosis, is expressed. We investigated the behaviour of these cells on a collagen matrix in vitro. These cells grew in a monolayer over foci of collagen degradation and could invade the collagen gel at such sites. Since the behavior of cell line AM-1 mimics the behavior of ameloblastoma in vivo, it may be a valuable model for the study of these neoplasms.
| Original language | English |
|---|---|
| Pages (from-to) | 207-212 |
| Number of pages | 6 |
| Journal | Journal of Oral Pathology and Medicine |
| Volume | 27 |
| Issue number | 5 |
| Publication status | Published - 1998 May |
| Externally published | Yes |
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Keywords
- Ameloblastoma
- Cell line
- Human papillomavirus
ASJC Scopus subject areas
- Cancer Research
- Pathology and Forensic Medicine
- Dentistry(all)
Cite this
Establishment of ameloblastoma cell line, AM-1. / Harada, Hidemitsu; Mitsuyasu, Takeshi; Nakamura, Norifumi; Higuchi, Yoshinori; Toyoshima, Kuniaki; Taniguchi, Akiyoshi; Yasumoto, Shigeru.
In: Journal of Oral Pathology and Medicine, Vol. 27, No. 5, 05.1998, p. 207-212.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Establishment of ameloblastoma cell line, AM-1
AU - Harada, Hidemitsu
AU - Mitsuyasu, Takeshi
AU - Nakamura, Norifumi
AU - Higuchi, Yoshinori
AU - Toyoshima, Kuniaki
AU - Taniguchi, Akiyoshi
AU - Yasumoto, Shigeru
PY - 1998/5
Y1 - 1998/5
N2 - Ameloblastomas are slowly growing, locally invasive neoplasms with a potentially destructive behaviour. The molecular mechanisms that regulate the cell growth and invasion of ameloblastoma cells are unknown. Because ameloblastoma cells placed in culture have a very limited lifespan, the establishment of immortalized clones of ameloblastoma cells would aid its study. We produced an immortalized ameloblastoma cell line (AM-1) using human papillomavirus type-16. This cell line maintains epithelial cell morphology and expresses cytokeratins K8, K14, K18, K19. Furthermore, bcl-2 protein, which prevents apoptosis, is expressed. We investigated the behaviour of these cells on a collagen matrix in vitro. These cells grew in a monolayer over foci of collagen degradation and could invade the collagen gel at such sites. Since the behavior of cell line AM-1 mimics the behavior of ameloblastoma in vivo, it may be a valuable model for the study of these neoplasms.
AB - Ameloblastomas are slowly growing, locally invasive neoplasms with a potentially destructive behaviour. The molecular mechanisms that regulate the cell growth and invasion of ameloblastoma cells are unknown. Because ameloblastoma cells placed in culture have a very limited lifespan, the establishment of immortalized clones of ameloblastoma cells would aid its study. We produced an immortalized ameloblastoma cell line (AM-1) using human papillomavirus type-16. This cell line maintains epithelial cell morphology and expresses cytokeratins K8, K14, K18, K19. Furthermore, bcl-2 protein, which prevents apoptosis, is expressed. We investigated the behaviour of these cells on a collagen matrix in vitro. These cells grew in a monolayer over foci of collagen degradation and could invade the collagen gel at such sites. Since the behavior of cell line AM-1 mimics the behavior of ameloblastoma in vivo, it may be a valuable model for the study of these neoplasms.
KW - Ameloblastoma
KW - Cell line
KW - Human papillomavirus
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M3 - Article
VL - 27
SP - 207
EP - 212
JO - Journal of Oral Pathology and Medicine
JF - Journal of Oral Pathology and Medicine
SN - 0904-2512
IS - 5
ER -