Abstract
Ameloblastomas are slowly growing, locally invasive neoplasms with a potentially destructive behaviour. The molecular mechanisms that regulate the cell growth and invasion of ameloblastoma cells are unknown. Because ameloblastoma cells placed in culture have a very limited lifespan, the establishment of immortalized clones of ameloblastoma cells would aid its study. We produced an immortalized ameloblastoma cell line (AM-1) using human papillomavirus type-16. This cell line maintains epithelial cell morphology and expresses cytokeratins K8, K14, K18, K19. Furthermore, bcl-2 protein, which prevents apoptosis, is expressed. We investigated the behaviour of these cells on a collagen matrix in vitro. These cells grew in a monolayer over foci of collagen degradation and could invade the collagen gel at such sites. Since the behavior of cell line AM-1 mimics the behavior of ameloblastoma in vivo, it may be a valuable model for the study of these neoplasms.
Original language | English |
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Pages (from-to) | 207-212 |
Number of pages | 6 |
Journal | Journal of Oral Pathology and Medicine |
Volume | 27 |
Issue number | 5 |
Publication status | Published - 1998 May 1 |
Externally published | Yes |
Keywords
- Ameloblastoma
- Cell line
- Human papillomavirus
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Oral Surgery
- Otorhinolaryngology
- Cancer Research
- Periodontics