Establishment of ameloblastoma cell line, AM-1

Hidemitsu Harada, Takeshi Mitsuyasu, Norifumi Nakamura, Yoshinori Higuchi, Kuniaki Toyoshima, Akiyoshi Taniguchi, Shigeru Yasumoto

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Ameloblastomas are slowly growing, locally invasive neoplasms with a potentially destructive behaviour. The molecular mechanisms that regulate the cell growth and invasion of ameloblastoma cells are unknown. Because ameloblastoma cells placed in culture have a very limited lifespan, the establishment of immortalized clones of ameloblastoma cells would aid its study. We produced an immortalized ameloblastoma cell line (AM-1) using human papillomavirus type-16. This cell line maintains epithelial cell morphology and expresses cytokeratins K8, K14, K18, K19. Furthermore, bcl-2 protein, which prevents apoptosis, is expressed. We investigated the behaviour of these cells on a collagen matrix in vitro. These cells grew in a monolayer over foci of collagen degradation and could invade the collagen gel at such sites. Since the behavior of cell line AM-1 mimics the behavior of ameloblastoma in vivo, it may be a valuable model for the study of these neoplasms.

Original languageEnglish
Pages (from-to)207-212
Number of pages6
JournalJournal of Oral Pathology and Medicine
Volume27
Issue number5
Publication statusPublished - 1998 May
Externally publishedYes

Fingerprint

Ameloblastoma
Cell Line
Collagen
Human papillomavirus 16
Keratins
Neoplasms
Clone Cells
Gels
Epithelial Cells
Apoptosis
Growth
Proteins

Keywords

  • Ameloblastoma
  • Cell line
  • Human papillomavirus

ASJC Scopus subject areas

  • Cancer Research
  • Pathology and Forensic Medicine
  • Dentistry(all)

Cite this

Harada, H., Mitsuyasu, T., Nakamura, N., Higuchi, Y., Toyoshima, K., Taniguchi, A., & Yasumoto, S. (1998). Establishment of ameloblastoma cell line, AM-1. Journal of Oral Pathology and Medicine, 27(5), 207-212.

Establishment of ameloblastoma cell line, AM-1. / Harada, Hidemitsu; Mitsuyasu, Takeshi; Nakamura, Norifumi; Higuchi, Yoshinori; Toyoshima, Kuniaki; Taniguchi, Akiyoshi; Yasumoto, Shigeru.

In: Journal of Oral Pathology and Medicine, Vol. 27, No. 5, 05.1998, p. 207-212.

Research output: Contribution to journalArticle

Harada, H, Mitsuyasu, T, Nakamura, N, Higuchi, Y, Toyoshima, K, Taniguchi, A & Yasumoto, S 1998, 'Establishment of ameloblastoma cell line, AM-1', Journal of Oral Pathology and Medicine, vol. 27, no. 5, pp. 207-212.
Harada H, Mitsuyasu T, Nakamura N, Higuchi Y, Toyoshima K, Taniguchi A et al. Establishment of ameloblastoma cell line, AM-1. Journal of Oral Pathology and Medicine. 1998 May;27(5):207-212.
Harada, Hidemitsu ; Mitsuyasu, Takeshi ; Nakamura, Norifumi ; Higuchi, Yoshinori ; Toyoshima, Kuniaki ; Taniguchi, Akiyoshi ; Yasumoto, Shigeru. / Establishment of ameloblastoma cell line, AM-1. In: Journal of Oral Pathology and Medicine. 1998 ; Vol. 27, No. 5. pp. 207-212.
@article{7cb78d19ead64638a9b669a18d2242c5,
title = "Establishment of ameloblastoma cell line, AM-1",
abstract = "Ameloblastomas are slowly growing, locally invasive neoplasms with a potentially destructive behaviour. The molecular mechanisms that regulate the cell growth and invasion of ameloblastoma cells are unknown. Because ameloblastoma cells placed in culture have a very limited lifespan, the establishment of immortalized clones of ameloblastoma cells would aid its study. We produced an immortalized ameloblastoma cell line (AM-1) using human papillomavirus type-16. This cell line maintains epithelial cell morphology and expresses cytokeratins K8, K14, K18, K19. Furthermore, bcl-2 protein, which prevents apoptosis, is expressed. We investigated the behaviour of these cells on a collagen matrix in vitro. These cells grew in a monolayer over foci of collagen degradation and could invade the collagen gel at such sites. Since the behavior of cell line AM-1 mimics the behavior of ameloblastoma in vivo, it may be a valuable model for the study of these neoplasms.",
keywords = "Ameloblastoma, Cell line, Human papillomavirus",
author = "Hidemitsu Harada and Takeshi Mitsuyasu and Norifumi Nakamura and Yoshinori Higuchi and Kuniaki Toyoshima and Akiyoshi Taniguchi and Shigeru Yasumoto",
year = "1998",
month = "5",
language = "English",
volume = "27",
pages = "207--212",
journal = "Journal of Oral Pathology and Medicine",
issn = "0904-2512",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Establishment of ameloblastoma cell line, AM-1

AU - Harada, Hidemitsu

AU - Mitsuyasu, Takeshi

AU - Nakamura, Norifumi

AU - Higuchi, Yoshinori

AU - Toyoshima, Kuniaki

AU - Taniguchi, Akiyoshi

AU - Yasumoto, Shigeru

PY - 1998/5

Y1 - 1998/5

N2 - Ameloblastomas are slowly growing, locally invasive neoplasms with a potentially destructive behaviour. The molecular mechanisms that regulate the cell growth and invasion of ameloblastoma cells are unknown. Because ameloblastoma cells placed in culture have a very limited lifespan, the establishment of immortalized clones of ameloblastoma cells would aid its study. We produced an immortalized ameloblastoma cell line (AM-1) using human papillomavirus type-16. This cell line maintains epithelial cell morphology and expresses cytokeratins K8, K14, K18, K19. Furthermore, bcl-2 protein, which prevents apoptosis, is expressed. We investigated the behaviour of these cells on a collagen matrix in vitro. These cells grew in a monolayer over foci of collagen degradation and could invade the collagen gel at such sites. Since the behavior of cell line AM-1 mimics the behavior of ameloblastoma in vivo, it may be a valuable model for the study of these neoplasms.

AB - Ameloblastomas are slowly growing, locally invasive neoplasms with a potentially destructive behaviour. The molecular mechanisms that regulate the cell growth and invasion of ameloblastoma cells are unknown. Because ameloblastoma cells placed in culture have a very limited lifespan, the establishment of immortalized clones of ameloblastoma cells would aid its study. We produced an immortalized ameloblastoma cell line (AM-1) using human papillomavirus type-16. This cell line maintains epithelial cell morphology and expresses cytokeratins K8, K14, K18, K19. Furthermore, bcl-2 protein, which prevents apoptosis, is expressed. We investigated the behaviour of these cells on a collagen matrix in vitro. These cells grew in a monolayer over foci of collagen degradation and could invade the collagen gel at such sites. Since the behavior of cell line AM-1 mimics the behavior of ameloblastoma in vivo, it may be a valuable model for the study of these neoplasms.

KW - Ameloblastoma

KW - Cell line

KW - Human papillomavirus

UR - http://www.scopus.com/inward/record.url?scp=0032077706&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032077706&partnerID=8YFLogxK

M3 - Article

VL - 27

SP - 207

EP - 212

JO - Journal of Oral Pathology and Medicine

JF - Journal of Oral Pathology and Medicine

SN - 0904-2512

IS - 5

ER -