Estimating the number of double-strand breaks formed during meiosis from partial observation

Hiroshi Toyoizumi, Hideo Tsubouchi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Analyzing the basic mechanism of DNA double-strand breaks (DSB) formation during meiosis is important for understanding sexual reproduction and genetic diversity. The location and amount of meiotic DSBs can be examined by using a common molecular biological technique called Southern blotting, but only a subset of the total DSBs can be observed; only DSB fragments still carrying the region recognized by a Southern blot probe are detected. With the assumption that DSB formation follows a nonhomogeneous Poisson process, we propose two estimators of the total number of DSBs on a chromosome: (1) an estimator based on the Nelson-Aalen estimator, and (2) an estimator based on a record value process. Further, we compared their asymptotic accuracy.

Original languageEnglish
Pages (from-to)1277-1283
Number of pages7
JournalJournal of Computational Biology
Volume19
Issue number12
DOIs
Publication statusPublished - 2012 Dec 1

Keywords

  • Double-strand breaks
  • Nelson-Aalen estimator
  • nonhomogeneous Poisson process
  • record value process southern blotting

ASJC Scopus subject areas

  • Modelling and Simulation
  • Molecular Biology
  • Genetics
  • Computational Mathematics
  • Computational Theory and Mathematics

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