Estimation of photosynthesis in cyanobacteria by pulse-amplitude modulation chlorophyll fluorescence: problems and solutions

Takako Ogawa, Masahiro Misumi, Kintake Sonoike

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Cyanobacteria are photosynthetic prokaryotes and widely used for photosynthetic research as model organisms. Partly due to their prokaryotic nature, however, estimation of photosynthesis by chlorophyll fluorescence measurements is sometimes problematic in cyanobacteria. For example, plastoquinone pool is reduced in the dark-acclimated samples in many cyanobacterial species so that conventional protocol developed for land plants cannot be directly applied for cyanobacteria. Even for the estimation of the simplest chlorophyll fluorescence parameter, Fv/Fm, some additional protocol such as addition of DCMU or illumination of weak blue light is necessary. In this review, those problems in the measurements of chlorophyll fluorescence in cyanobacteria are introduced, and solutions to those problems are given.

    Original languageEnglish
    Pages (from-to)1-11
    Number of pages11
    JournalPhotosynthesis Research
    DOIs
    Publication statusAccepted/In press - 2017 Mar 10

    Fingerprint

    Pulse amplitude modulation
    Photosynthesis
    Cyanobacteria
    Chlorophyll
    Fluorescence
    photosynthesis
    fluorescence
    chlorophyll
    Plastoquinone
    Embryophyta
    Diuron
    embryophytes
    blue light
    prokaryotic cells
    Lighting
    lighting
    Light
    organisms
    Research
    sampling

    Keywords

    • Chlorophyll fluorescence
    • Cyanobacteria
    • Nonphotochemical quenching
    • Pulse-amplitude modulation (PAM) fluorometry
    • State transition

    ASJC Scopus subject areas

    • Biochemistry
    • Plant Science
    • Cell Biology

    Cite this

    Estimation of photosynthesis in cyanobacteria by pulse-amplitude modulation chlorophyll fluorescence : problems and solutions. / Ogawa, Takako; Misumi, Masahiro; Sonoike, Kintake.

    In: Photosynthesis Research, 10.03.2017, p. 1-11.

    Research output: Contribution to journalArticle

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