Abstract
Cyanobacteria are photosynthetic prokaryotes and widely used for photosynthetic research as model organisms. Partly due to their prokaryotic nature, however, estimation of photosynthesis by chlorophyll fluorescence measurements is sometimes problematic in cyanobacteria. For example, plastoquinone pool is reduced in the dark-acclimated samples in many cyanobacterial species so that conventional protocol developed for land plants cannot be directly applied for cyanobacteria. Even for the estimation of the simplest chlorophyll fluorescence parameter, Fv/Fm, some additional protocol such as addition of DCMU or illumination of weak blue light is necessary. In this review, those problems in the measurements of chlorophyll fluorescence in cyanobacteria are introduced, and solutions to those problems are given.
| Original language | English |
|---|---|
| Pages (from-to) | 1-11 |
| Number of pages | 11 |
| Journal | Photosynthesis Research |
| DOIs | |
| Publication status | Accepted/In press - 2017 Mar 10 |
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Keywords
- Chlorophyll fluorescence
- Cyanobacteria
- Nonphotochemical quenching
- Pulse-amplitude modulation (PAM) fluorometry
- State transition
ASJC Scopus subject areas
- Biochemistry
- Plant Science
- Cell Biology
Cite this
Estimation of photosynthesis in cyanobacteria by pulse-amplitude modulation chlorophyll fluorescence : problems and solutions. / Ogawa, Takako; Misumi, Masahiro; Sonoike, Kintake.
In: Photosynthesis Research, 10.03.2017, p. 1-11.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Estimation of photosynthesis in cyanobacteria by pulse-amplitude modulation chlorophyll fluorescence
T2 - problems and solutions
AU - Ogawa, Takako
AU - Misumi, Masahiro
AU - Sonoike, Kintake
PY - 2017/3/10
Y1 - 2017/3/10
N2 - Cyanobacteria are photosynthetic prokaryotes and widely used for photosynthetic research as model organisms. Partly due to their prokaryotic nature, however, estimation of photosynthesis by chlorophyll fluorescence measurements is sometimes problematic in cyanobacteria. For example, plastoquinone pool is reduced in the dark-acclimated samples in many cyanobacterial species so that conventional protocol developed for land plants cannot be directly applied for cyanobacteria. Even for the estimation of the simplest chlorophyll fluorescence parameter, Fv/Fm, some additional protocol such as addition of DCMU or illumination of weak blue light is necessary. In this review, those problems in the measurements of chlorophyll fluorescence in cyanobacteria are introduced, and solutions to those problems are given.
AB - Cyanobacteria are photosynthetic prokaryotes and widely used for photosynthetic research as model organisms. Partly due to their prokaryotic nature, however, estimation of photosynthesis by chlorophyll fluorescence measurements is sometimes problematic in cyanobacteria. For example, plastoquinone pool is reduced in the dark-acclimated samples in many cyanobacterial species so that conventional protocol developed for land plants cannot be directly applied for cyanobacteria. Even for the estimation of the simplest chlorophyll fluorescence parameter, Fv/Fm, some additional protocol such as addition of DCMU or illumination of weak blue light is necessary. In this review, those problems in the measurements of chlorophyll fluorescence in cyanobacteria are introduced, and solutions to those problems are given.
KW - Chlorophyll fluorescence
KW - Cyanobacteria
KW - Nonphotochemical quenching
KW - Pulse-amplitude modulation (PAM) fluorometry
KW - State transition
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UR - http://www.scopus.com/inward/citedby.url?scp=85014799345&partnerID=8YFLogxK
U2 - 10.1007/s11120-017-0367-x
DO - 10.1007/s11120-017-0367-x
M3 - Article
C2 - 28283890
AN - SCOPUS:85014799345
SP - 1
EP - 11
JO - Photosynthesis Research
JF - Photosynthesis Research
SN - 0166-8595
ER -