Estimation of photosynthesis in cyanobacteria by pulse-amplitude modulation chlorophyll fluorescence: problems and solutions

Takako Ogawa, Masahiro Misumi, Kintake Sonoike*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

27 Citations (Scopus)

Abstract

Cyanobacteria are photosynthetic prokaryotes and widely used for photosynthetic research as model organisms. Partly due to their prokaryotic nature, however, estimation of photosynthesis by chlorophyll fluorescence measurements is sometimes problematic in cyanobacteria. For example, plastoquinone pool is reduced in the dark-acclimated samples in many cyanobacterial species so that conventional protocol developed for land plants cannot be directly applied for cyanobacteria. Even for the estimation of the simplest chlorophyll fluorescence parameter, Fv/Fm, some additional protocol such as addition of DCMU or illumination of weak blue light is necessary. In this review, those problems in the measurements of chlorophyll fluorescence in cyanobacteria are introduced, and solutions to those problems are given.

Original languageEnglish
Pages (from-to)63-73
Number of pages11
JournalPhotosynthesis Research
Volume133
Issue number1-3
DOIs
Publication statusPublished - 2017 Sep 1

Keywords

  • Chlorophyll fluorescence
  • Cyanobacteria
  • Nonphotochemical quenching
  • Pulse-amplitude modulation (PAM) fluorometry
  • State transition

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

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