TY - JOUR
T1 - Estimation of photosynthesis in cyanobacteria by pulse-amplitude modulation chlorophyll fluorescence
T2 - problems and solutions
AU - Ogawa, Takako
AU - Misumi, Masahiro
AU - Sonoike, Kintake
N1 - Funding Information:
This work was supported by JSPS Grant-in-Aid for Scientific Research on Innovative Areas (No. 16H06552 and No. 16H06553 to K.S.) and Grant-in-Aid for Scientific Research (B) (No. 16H04809 to K.S.), as well as by Grant-in-Aid for JSPS Research Fellow (No. 26-7221 to T.O.). We thank Dr. Yukako Hihara for the critical reading of the manuscript. Takako Ogawa and Masahiro Misumi have contributed equally to this work.
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Cyanobacteria are photosynthetic prokaryotes and widely used for photosynthetic research as model organisms. Partly due to their prokaryotic nature, however, estimation of photosynthesis by chlorophyll fluorescence measurements is sometimes problematic in cyanobacteria. For example, plastoquinone pool is reduced in the dark-acclimated samples in many cyanobacterial species so that conventional protocol developed for land plants cannot be directly applied for cyanobacteria. Even for the estimation of the simplest chlorophyll fluorescence parameter, Fv/Fm, some additional protocol such as addition of DCMU or illumination of weak blue light is necessary. In this review, those problems in the measurements of chlorophyll fluorescence in cyanobacteria are introduced, and solutions to those problems are given.
AB - Cyanobacteria are photosynthetic prokaryotes and widely used for photosynthetic research as model organisms. Partly due to their prokaryotic nature, however, estimation of photosynthesis by chlorophyll fluorescence measurements is sometimes problematic in cyanobacteria. For example, plastoquinone pool is reduced in the dark-acclimated samples in many cyanobacterial species so that conventional protocol developed for land plants cannot be directly applied for cyanobacteria. Even for the estimation of the simplest chlorophyll fluorescence parameter, Fv/Fm, some additional protocol such as addition of DCMU or illumination of weak blue light is necessary. In this review, those problems in the measurements of chlorophyll fluorescence in cyanobacteria are introduced, and solutions to those problems are given.
KW - Chlorophyll fluorescence
KW - Cyanobacteria
KW - Nonphotochemical quenching
KW - Pulse-amplitude modulation (PAM) fluorometry
KW - State transition
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U2 - 10.1007/s11120-017-0367-x
DO - 10.1007/s11120-017-0367-x
M3 - Review article
C2 - 28283890
AN - SCOPUS:85014799345
VL - 133
SP - 63
EP - 73
JO - Photosynthesis Research
JF - Photosynthesis Research
SN - 0166-8595
IS - 1-3
ER -