Evaluation of a centrifuged double Y-shape microfluidic platform for simple continuous cell environment exchange

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have demonstrated the efficacy of a microfluidic medium exchange method for single cells using passive centrifugal force of a rotating microfluidic-chip based platform. At the boundary of two laminar flows at the gathering area of two microfluidic pathways in a Y-shape, the cells were successfully transported from one laminar flow to the other, without mixing the two microfluidic mediums of the two laminar flows during cell transportation, within 5 s with 1 g (150 rpm) to 36.3 g (900 rpm) acceleration, with 93.5% efficiency. The results indicate that this is one of the most simple and precise tools for exchanging medium in the shortest amount of time.

Original languageEnglish
Pages (from-to)819-827
Number of pages9
JournalInternational Journal of Molecular Sciences
Volume13
Issue number1
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Microfluidics
laminar flow
platforms
Laminar flow
evaluation
cells
centrifugal force
exchanging
Cell Shape
chips

Keywords

  • Centrifugal force
  • Environmental control
  • Medium exchange
  • Microfluidic chip
  • On-chip cellomics
  • Single cell
  • Y-shape platform

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Medicine(all)

Cite this

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abstract = "We have demonstrated the efficacy of a microfluidic medium exchange method for single cells using passive centrifugal force of a rotating microfluidic-chip based platform. At the boundary of two laminar flows at the gathering area of two microfluidic pathways in a Y-shape, the cells were successfully transported from one laminar flow to the other, without mixing the two microfluidic mediums of the two laminar flows during cell transportation, within 5 s with 1 g (150 rpm) to 36.3 g (900 rpm) acceleration, with 93.5{\%} efficiency. The results indicate that this is one of the most simple and precise tools for exchanging medium in the shortest amount of time.",
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AU - Yasuda, Kenji

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AB - We have demonstrated the efficacy of a microfluidic medium exchange method for single cells using passive centrifugal force of a rotating microfluidic-chip based platform. At the boundary of two laminar flows at the gathering area of two microfluidic pathways in a Y-shape, the cells were successfully transported from one laminar flow to the other, without mixing the two microfluidic mediums of the two laminar flows during cell transportation, within 5 s with 1 g (150 rpm) to 36.3 g (900 rpm) acceleration, with 93.5% efficiency. The results indicate that this is one of the most simple and precise tools for exchanging medium in the shortest amount of time.

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