TY - JOUR
T1 - Evaluation of antibacterial activity of zinc-doped hydroxyapatite colloids and dispersion stability using ultrasounds
AU - Predoi, Daniela
AU - Iconaru, Simona Liliana
AU - Predoi, Mihai Valentin
AU - Motelica-Heino, Mikael
AU - Guegan, Regis
AU - Buton, Nicolas
N1 - Funding Information:
Funding: This research was partially funded by the Romanian Ministry of Research and Innovation with the project number PN-III-P1-1.2-PCCDI-2017-0629/contract no. 43PCCDI/2018.
Funding Information:
Acknowledgments: We thank A.R. from the “Centre de Microscopie Electronique” of the University of Orléans for assistance in SEM data acquisition and A.M.P. and M.L.B. for assistance with the in vitro experiments. This research was funded by the Romanian Ministry of Research and Innovation PCCDI–UEFISCDI, through the projects PN-III-P1-1.2-PCCDI-2017-0629, Contract No. 43PCCDI/2018, and Core Program 21N/2019.
Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2019/4
Y1 - 2019/4
N2 - This study proves that the new developed zinc-doped hydroxyapatite (ZnHAp) colloids by an adapted sol-gel method can be widely used in the pharmaceutical, medical, and environmental industries. ZnHAp nanoparticles were stabilized in an aqueous solution, and their colloidal dispersions have been characterized by different techniques. Scanning Electron Microscopy (SEM) was used to get information on the morphology and composition of the investigated samples. Energy-dispersive X-ray spectroscopy (EDX) analysis confirmed the elemental compositions of ZnHAp colloidal dispersions. The homogeneous and uniform distribution of constituent elements (zinc, calcium, phosphorus, oxygen) was highlighted by the obtained elemental mapping results. The X-ray diffraction (XRD) results of the obtained samples showed a single phase corresponding to the hexagonal hydroxyapatite. The characteristic bands of the hydroxyapatite structure were also evidenced by Fourier-transform infrared spectroscopy (FTIR) analysis. For a stability assessment of the colloidal system, ζ-potential for the ZnHAp dispersions was estimated. Dynamic light scattering (DLS) was used to determine particles dispersion and hydrodynamic diameter (D HYD ). The goal of this study was to provide for the first time information on the stability of ZnHAp particles in solutions evaluated by non-destructive ultrasound-based technique. In this work, the influence of the ZnHAp colloidal solutions stability on the development of bacteria, such as Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), was also established for the first time. The antimicrobial activity of ZnHAp solutions was strongly influenced by both the stability of the solutions and the amount of Zn.
AB - This study proves that the new developed zinc-doped hydroxyapatite (ZnHAp) colloids by an adapted sol-gel method can be widely used in the pharmaceutical, medical, and environmental industries. ZnHAp nanoparticles were stabilized in an aqueous solution, and their colloidal dispersions have been characterized by different techniques. Scanning Electron Microscopy (SEM) was used to get information on the morphology and composition of the investigated samples. Energy-dispersive X-ray spectroscopy (EDX) analysis confirmed the elemental compositions of ZnHAp colloidal dispersions. The homogeneous and uniform distribution of constituent elements (zinc, calcium, phosphorus, oxygen) was highlighted by the obtained elemental mapping results. The X-ray diffraction (XRD) results of the obtained samples showed a single phase corresponding to the hexagonal hydroxyapatite. The characteristic bands of the hydroxyapatite structure were also evidenced by Fourier-transform infrared spectroscopy (FTIR) analysis. For a stability assessment of the colloidal system, ζ-potential for the ZnHAp dispersions was estimated. Dynamic light scattering (DLS) was used to determine particles dispersion and hydrodynamic diameter (D HYD ). The goal of this study was to provide for the first time information on the stability of ZnHAp particles in solutions evaluated by non-destructive ultrasound-based technique. In this work, the influence of the ZnHAp colloidal solutions stability on the development of bacteria, such as Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), was also established for the first time. The antimicrobial activity of ZnHAp solutions was strongly influenced by both the stability of the solutions and the amount of Zn.
KW - Antibacterial activity
KW - Nanoparticles
KW - Ultrasound technique
KW - Zinc-doped hydroxyapatite
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U2 - 10.3390/nano9040515
DO - 10.3390/nano9040515
M3 - Article
AN - SCOPUS:85065498732
VL - 9
JO - Nanomaterials
JF - Nanomaterials
SN - 2079-4991
IS - 4
M1 - 515
ER -