Evaluation of blood lactate and plasma insulin during high-intensity exercise by antecubital vein catheterization

Minas Nalbandian, Zsolt Radak, Masaki Takeda

Research output: Contribution to journalArticlepeer-review

Abstract

The measurement of metabolic and endocrinal markers during physical activity is of relevance to understanding the physiological implications of different exercise modalities. During some exercise modalities (e.g., high-intensity interval exercise), blood metabolites and hormonal levels change in short periods of time. In the present study, we describe a method to catheterize the antecubital vein, which allows the collection of several blood samples during exercise. Insulin and venous lactate concentrations were measured during high-intensity exercise by the application of the described method. The exercise consisted of three 30 s bouts of high-intensity exercise separated by 4 min of recovery. After the last recovery period, a Wingate test was performed. Blood samples from the antecubital vein were obtained before and after each 30 s bout and before and after the Wingate test. As a result, it was possible to evaluate the plasma insulin and venous blood lactate variations during the exercise.

Original languageEnglish
Article numbere56890
JournalJournal of Visualized Experiments
Volume2018
Issue number135
DOIs
Publication statusPublished - 2018 May 18
Externally publishedYes

Keywords

  • Antecubital vein catheterization
  • Blood sampling
  • Exercise testing
  • High-intensity interval exercise
  • Hormones
  • Immunology and Infection
  • Issue 135
  • Wingate test

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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